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German Centre for Cardiovascular Research (DZHK), Munich Heart Alliance

1 ARTICLES PUBLISHED IN JoVE

Biology

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

Alexander Goedel^*1,2, Dorota M. Zawada^*1, Fangfang Zhang¹, Zhifen Chen³, Alessandra Moretti^1,2, Daniel Sinnecker^1,2

¹Medical Department I, University Hospital Klinikum rechts der Isar, Technical University of Munich, ²German Centre for Cardiovascular Research (DZHK), Munich Heart Alliance, ³Beth Israel Deaconess Medical Center, Harvard Medical School

Here we present a method to optically image action potentials, specifically in ventricular-like induced pluripotent stem cell-derived cardiomyocytes. The method is based on the promoter-driven expression of a voltage-sensitive fluorescent protein.