Covalent Immobilization of Proteins for the Single Molecule Force SpectroscopyTanja D. Becke 1,2,3, Stefan Ness 2, Stefanie Sudhop 1,3, Hermann E. Gaub 3, Markus Hilleringmann 2, Arndt F. Schilling *4, Hauke Clausen-Schaumann *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
This protocol describes the covalent immobilization of proteins with a heterobifunctional silane coupling agent to silicon-oxide surfaces designed for the atomic force microscopy based single molecule force spectroscopy which is exemplified by the interaction of RrgA (pilus-1 tip adhesin of S. pneumoniae) with fibronectin.
