Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem CellsKarim Ben M'Barek 1,2,3, Walter Habeler 1,2,3, Alexandra Plancheron 1,2,3, Mohamed Jarraya 4, Olivier Goureau 5, Christelle Monville 1,2
1U861, I-Stem, Association Française contre les Myopathies (AFM), Institut National de la Santé et de la Recherche Médicale (INSERM), 2U861, I-Stem, Association Française contre les Myopathies (AFM), Université Evry Val-d'Essonne (UEVE), 3I-Stem, Association Française contre les Myopathies (AFM), Centre pour L’Etude des Cellules Souches (CECS), 4Banque de tissus humain, Hôpital Saint Louis, Assistance Publique - Hôpitaux de Paris (AP-HP), 5Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012
We describe a method to engineer a retinal tissue composed of retinal pigment epithelial cells derived from human pluripotent stem cells cultured on top of human amniotic membranes and its preparation for grafting in animal models.
