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Oryzon Genomics

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Direct Measurement of KDM1A Target Engagement Using Chemoprobe-based Immunoassays

Cristina Mascaró¹, Raquel Ruiz Rodriguez¹, Tamara Maes¹

Here, we present a protocol to measure KDM1A target engagement in a human or animal cell, tissue or blood samples treated with KDM1A inhibitors. The protocol employs chemoprobe tagging of the free KDM1A enzyme and direct quantification of the target occupation using chemoprobe-based immunoassays and can be used in preclinical and clinical studies.