Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular ResolutionLeighton H. Duncan 1,5, Mark W. Moyle 1,5, Lin Shao 1,5, Titas Sengupta 1,5, Richard Ikegami 1,5, Abhishek Kumar 4,5, Min Guo 4,5, Ryan Christensen 4,5, Anthony Santella 2,5, Zhirong Bao 2,5, Hari Shroff 4,5, William Mohler 3,5, Daniel A. Colón-Ramos 1,5,6
1Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine, 2Developmental Biology Program, Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling, University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico
Here, we present a combinatorial approach using high-resolution microscopy, computational tools, and single-cell labeling in living C. elegans embryos to understand single cell dynamics during neurodevelopment.
