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Centro Hospitalar Universitário Lisboa Norte

3 ARTICLES PUBLISHED IN JoVE

Medicine

Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia

Augusto Ministro^1,2, Paula de Oliveira¹, Raquel J. Nunes¹, André dos Santos Rocha¹, Tiago Ferreira², J. Goyri-O'Neill³, Susana Constantino Rosa Santos¹

¹Centro Cardiovascular da Universidade de Lisboa, Lisbon School of Medicine of the Universidade de Lisboa, ²Centro Hospitalar Universitário Lisboa Norte, ³Nova Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa

Here, a critical hindlimb ischemia experimental model is presented followed by a battery of functional, histologic and molecular tests to assess the effectiveness of angiogenic therapies.

Medicine

Echocardiographic Assessment of Cardiac Anatomy and Function in Adult Rats

Sónia Ribeiro^*1,2, Ana Rita S. Pereira^*1, Ana Teresa Pinto¹, Filipe Rocha¹, Augusto Ministro^1,2, Manuela Fiuza^1,2, Fausto Pinto^1,2, Susana Constantino Rosa Santos¹

¹Centro Cardiovascular da Universidade de Lisboa, Faculdade de Medicina, Universidade de Lisboa, ²Departamento Coração e Vasos, Centro Hospitalar Universitário Lisboa Norte

A non-invasive protocol for transthoracic echocardiography assessment of cardiac anatomy and function for adult rats is presented in the current study. The heart valves, all four cardiac chambers and the ascending aorta, aortic arch and descending aorta are studied in detail.

Medicine

Urethral Stricture Induction Followed by Buccal Mucosa Graft Urethroplasty in a Rat Model

Pedro S. de Oliveira^1,2, Filipe Rocha¹, Inês Sofia Vala¹, Paula de Oliveira¹, Augusto Ministro^1,3, Susana Constantino Rosa Santos¹

¹Cardiovascular Center of the University of Lisbon (CCUL@RISE), Faculdade de Medicina da Universidade de Lisboa, ²Department of Urology, Centro Hospitalar Universitário Lisboa Norte, ³Department of Vascular Surgery, Centro Hospitalar Universitário Lisboa Norte

In the present protocol, a urethral stricture induction was developed in Wistar rats, followed by urethral reconstruction with a buccal mucosa graft. A retrograde urethrogram and laser Doppler assessment were performed, validating urethral reconstruction (after stricture formation) and graft placement.