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Biogenic Nanotherapeutics group (BION)

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Evaluation of the Storage Stability of Extracellular Vesicles

Maximilian Richter^1,2, Kathrin Fuhrmann¹, Gregor Fuhrmann^1,2

¹Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI), Biogenic Nanotherapeutics group (BION), ²Department of Pharmacy, Saarland University

Here we present a readily applicable protocol to assess the storage stability of extracellular vesicles, a group of naturally occurring nanoparticles produced by cells. The vesicles are loaded with glucuronidase as a model enzyme and stored under different conditions. After storage, their physicochemical parameters and the activity of the encapsulated enzyme are evaluated.