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Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital

1 ARTICLES PUBLISHED IN JoVE

Genetics

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Sampath Kumar Loganathan¹, Ahmad Malik^1,2, Ellen Langille^1,2, Chen Luxenburg³, Daniel Schramek^1,2

¹Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, ²Department of Molecular Genetics, University of Toronto, ³Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent mice.