Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid ModelsGeorgios Stroulios *1, Martin Stahl *2, Fisal Elstone *2, Wing Chang 1, Sharon Louis 2, Allen Eaves 2,3, Salvatore Simmini 1, Ryan K. Conder 2
1STEMCELL Technologies Ltd., 2STEMCELL Technologies Inc., 3Terry Fox Laboratory, BC Cancer
Here we present two protocols that allow the modeling of intestinal apical-specific interactions. Organoid-derived intestinal monolayers and Air-Liquid Interface (ALI) cultures facilitate the generation of well-differentiated epithelia accessible from both luminal and basolateral sides, whereas polarity-inverted intestinal organoids expose their apical side and are amenable to high throughput assays.
