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Morsani College of Medicine, University of South Florida

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model

¹Department of Molecular Medicine, Morsani College of Medicine, University of South Florida

Synthetic cystic fibrosis sputum medium (SCFM2) can be utilized in combination with both confocal laser scanning microscopy and fluorescence-activated cell sorting to observe bacterial aggregates at high resolution. This paper details methods to assess aggregate populations during antimicrobial treatment as a platform for future studies.