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National Institute of Metrology, Quality and Technology (Inmetro)

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

Gabriela S. Kronemberger^1,2,3, Guilherme A. S. C. Miranda^1,2,4, Taisnara I. G. Silva^1,2,4, Rosângela M. Gonçalves^1,2, José M. Granjeiro^2,3,4,5, Leandra S. Baptista^1,2,3,4

¹Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, ²Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), ³Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, ⁴Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), ⁵Dental School, Fluminense Federal Fluminense

Here, we describe the large-scale production of adipose-derived stromal/stem cell (ASC) spheroids using an automated pipetting system to seed the cell suspension, thus ensuring homogeneity of spheroid size and shape. These ASC spheroids can be used as building blocks for 3D bioprinting approaches.