Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus MechanobiologyMiao Huang 1,5, Heyang Wang 1,5, Alfredo A. Delgado 1, Tyler A. Reid 1, Julian Long 2, Shu Wang 3,5, Hayley Sussman 4, Juan Guan 5,6,7, Hitomi Yamaguchi 1, Xin Tang 1,5,8,9
1Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Materials Science and Engineering, University of Florida, 3Department of Biostatistics, University of Florida, 4Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida, 8J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 9Department of Physiology and Functional Genomics, University of Florida
This study presents a new protocol to directly apply mechanical force on the cell nucleus through magnetic microbeads delivered into the cytoplasm and to conduct simultaneous live-cell fluorescent imaging.
