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Ludwig-Maximilians University

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Bob A. Hersbach^1,2,3, Tatiana Simon², Giacomo Masserdotti^1,2

¹Institute of Stem Cell Research, Helmholtz Zentrum München, German Research Center for Environmental Health, ²Department of Physiological Genomics, Biomedical Center Munich, Ludwig-Maximilians University, ³Graduate School of Systemic Neurosciences, BioCenter, Ludwig-Maximilians University

Here we describe a detailed protocol to generate highly enriched cultures of astrocytes derived from different regions of the central nervous system of postnatal mice and their direct conversion into functional neurons by the forced expression of transcription factors.