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Federal University of Paraná (UFPR)

2 ARTICLES PUBLISHED IN JoVE

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A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

Irisdoris Rodrigues de Souza¹, Andrezza Di Pietro Micali Canavez², Desiree Cigaran Schuck², Viviana Stephanie Costa Gagosian¹, Isisdoris Rodrigues de Souza¹, Natália de Albuquerque Vita², Edvaldo da Silva Trindade³, Marta Margarete Cestari⁴, Marcio Lorencini², Daniela Morais Leme⁴

¹Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), ²Safety of Product Department, Grupo Boticário, ³Department of Cell Biology, Federal University of Paraná (UFPR), ⁴Department of Genetics, Federal University of Paraná (UFPR)

Here, we present an effective, easy, and fast 3D culture protocol for the formation of spheroids of two zebrafish (Danio rerio) cell lines: ZEM2S (embryo) and ZFL (normal hepatocyte).

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Cytotoxicity Assays with Zebrafish Cell Lines

Irisdoris Rodrigues de Souza¹, Tainá Wilke Sivek¹, Júlia Beatriz Vaz de Oliveira¹, Andrezza Di Pietro Micali Canavez², Natália de Albuquerque Vita², Desiree Cigaran Schuck², Isisdoris Rodrigues de Souza¹, Marta Margarete Cestari³, Marcio Lorencini², Daniela Morais Leme³

¹Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), ²Safety of Product Department, Grupo Boticário, ³Department of Genetics, Federal University of Paraná (UFPR)

This protocol presents commonly used cytotoxicity assays (Alamar Blue [AB], CFDA-AM, Neutral Red, and MTT assays) adapted for the assessment of cytotoxicity in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates.