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European Institute of Oncology (IEO)

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Núria Crua Asensio¹, Stefano Rizzieri², Alessandro Cuomo², Gian Gaetano Tartaglia^1,3, Elsa Zacco¹

¹Center for Human Technology, Italian Institute of Technology (IIT), ²Department of Experimental Oncology, European Institute of Oncology (IEO), ³Department of Biology and Biotechnologies ‘Charles Darwin’, Sapienza University of Rome

Here, we present an optimized in vitro method to uncover, quantify, and validate protein interactors of specific RNA sequences, using total protein extract from human cells, streptavidin beads coated with biotinylated RNA, and mass spectrometry analysis.