biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

https://cloudfront.jove.com/files/eoe/neuroscience.jpg

jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

breast cancer

This collection features biomedical research methods employed in research laboratories to advance breast cancer prevention, detection, and treatment.

Breast Cancer

lung cancer

This collection highlights some of the latest protocols in lung cancer research. The collection covers techniques relating to the generation of lung cancer animal models, cancer cell characterization, treatment and detection strategies, in vitro culture studies, assays and isolation techniques that facilitate lung cancer research.

Lung Cancer

leukemia

Leukemia reflects cancer primarily resulting from an elevated number of white blood cells in the body. This collection features a set of in vitro assay techniques to analyze leukemic cell growth and metabolism, cell isolation and culture methods, approaches to characterize and study cellular morphology, and genetic manipulation procedures.

Leukemia

pancreatic cancer

The collection features protocols demonstrating the generation of pancreatic animal models, genetic engineering of pancreatic cancer cells, methods for in vitro modeling of pancreatic cancer, cell isolation & characterization techniques, and tumor cell behavior and response studies.

Pancreatic Cancer

colorectal cancer

Colorectal cancer originates within the colon or rectal regions of organisms. This collection highlights some fundamental research techniques in colorectal cancer relating to screening tests, genetic analysis, studies on the gut microbiome, therapeutic interventions, animal model generation, assay techniques, and cell behavior & metastatic responses.

Colorectal Cancer

skin cancer

This collection focusses on research approaches relating to melanoma and other types of skin cancer. The protocols feature techniques relevant to developing in vitro and in vivo skin carcinogenesis and metastatic models, cell culturing, skin regeneration approaches, genetic manipulation and sensitivity studies using skin cells, and assay techniques.

Skin Cancer

gynecologic cancer

This collection features protocols relating to the prevention, detection, and treatment of cancer originating from cells or regions of the female reproductive system. The protocols are relevant for cancer-targeting and detection approaches, metabolic assessment of cancer cells, developing in vivo and metastatic animal models, in vitro cell culture and assay techniques, and transplantation experiments.

Gynecologic Cancer

prostate cancer

This collection features techniques employed in prostate cancer research relating to cancer cell/organoid culture, processing, and staining, cell and tissue sampling, organ extractions, in vivo & in vitro cancer modeling, and cancer proteomics studies.

Prostate Cancer

gastrointestinal cancer

Gastrointestinal cancers include various tumor types originating in the regions or organs associated with the gastrointestinal tract. The collection comprises research protocols on cell isolation, culturing, and adhesion studies, tissue staining and histological analysis, DNA methylation studies, tissue preservation methods, exosome analysis, and generation of animal models.

Gastrointestinal Cancer

head and neck cancer

Head and neck cancers constitute carcinomas originating in and around the mucosal surfaces of the head and neck regions. This collection features protocols relating to cancer modeling in vitro, in ovo, and in vivo, cell isolation and culture, fluorescent in situ hybridization-related techniques, generation of decellularized tissue matrices, and drug-resistance characterization assays.

Head and Neck Cancer

urinary tract cancer

This collection features protocols relating to cancers originating in the regions or organs of the urinary or renal system. The techniques include organs, tissue, and cell harvesting procedures, organotypic culturing approaches, in vivo cancer modeling studies, decellularized tissue matrix generation, stem cell-related and electrophysiology studies, genetic-engineering strategies, tissue staining and imaging techniques, and protein expression analysis.

Urinary Tract Cancer

cancers of the nervous system

This collection features protocols relating to cancers originating in the nervous system. The techniques include intra-brain injections, in-vivo and in-vitro tumor modeling, cell and organelle culturing, extracellular vesicle harvesting and purification methodologies, proteomic analysis, organ isolation and preservation methods, cell migration assays, tissue engineering methods, and novel gene delivery approaches.

Cancers of the Nervous System

in vitro studies

In vitro studies

in vivo studies

In vivo studies

ex vivo studies

Ex vivo studies

exosome isolation: a density-based ultracentrifugation technique using sucrose cushion to separate exosomes from conditioned media of cultured cells

exosome-isolation-density-based-ultracentrifugation-technique-using

Exosome Isolation: A Density-based Ultracentrifugation Technique Using Sucrose Cushion to Separate Exosomes from Conditioned Media of Cultured Cells

Source: Faruqu, F. N. et al. Preparation of Exosomes for siRNA Delivery to Cancer Cells.&#160;J. Vis. Exp.&#160;(2018)
This video describes a ...

sirna encapsulation into exosomes using electroporation: an electric pulse based technique to load sirna into exosomes

sirna-encapsulation-into-exosomes-using-electroporation-an-electric

siRNA Encapsulation into Exosomes Using Electroporation: An Electric Pulse Based Technique to Load siRNA into Exosomes

Source: Faruqu, F. N. et al. Preparation of Exosomes for siRNA Delivery to Cancer Cells. J. Vis. Exp. (2018)
This video describes an electroporation-based ...

solid phase microextraction sampling: a probe-based technique for sample extraction from graft tissues

solid-phase-microextraction-sampling-probe-based-technique-for-sample

Solid Phase Microextraction Sampling: A Probe-based Technique for Sample Extraction from Graft Tissues

Source: Stryjak, I. et al. Using a Chemical Biopsy for Graft Quality Assessment. J. Vis. Exp. (2020)
In this video, we demonstrate SPME-probe-based ...

amphotericin-b mediated perforated patch-clamp analysis: an electrophysiological technique to study ionic currents in urinary bladder cells

amphotericin-b-mediated-perforated-patch-clamp-analysis-an

Amphotericin-B Mediated Perforated Patch-Clamp Analysis: An Electrophysiological Technique to Study Ionic Currents in Urinary Bladder Cells

Source: Malysz, J. et al. Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of ...

preparation of cancer spheroid blocks by flash freezing: a procedure to preserve cancer spheroids in frozen matrix blocks

preparation-cancer-spheroid-blocks-flash-freezing-procedure-to

Preparation of Cancer Spheroid Blocks by Flash Freezing: A Procedure To Preserve Cancer Spheroids in Frozen Matrix Blocks

Source: Wang, Y. et al. 3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer. J. Vis. Exp. (2018)
This video ...

deriving human kidney podocytes from induced pluripotent stem cells: a procedure for directed differentiation of mature kidney podocytes from stem cells

deriving-human-kidney-podocytes-from-induced-pluripotent-stem-cells

Deriving Human Kidney Podocytes from Induced Pluripotent Stem Cells: A Procedure for Directed Differentiation of Mature Kidney Podocytes from Stem Cells

Source: Burt, M. et al. Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions. ...

detrusor smooth muscle cell isolation: an enzymatic digestion procedure to obtain dsm cells from human urinary bladder specimens

detrusor-smooth-muscle-cell-isolation-an-enzymatic-digestion

Detrusor Smooth Muscle Cell Isolation: An Enzymatic Digestion Procedure to Obtain DSM Cells from Human Urinary Bladder Specimens

vibratome sectioning of whole murine kidney: a technique to generate thin sections of murine kidney

vibratome-sectioning-whole-murine-kidney-technique-to-generate-thin

Vibratome Sectioning of Whole Murine Kidney: A Technique to Generate Thin Sections of Murine Kidney

Source: Grainger, N. et al. Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning. J. Vis. Exp. (2021)
In ...

extracellular dna staining: a method to identify extracellular dna in ffpe tissue sections

extracellular-dna-staining-method-to-identify-extracellular-dna-ffpe

Extracellular DNA Staining: A Method to Identify Extracellular DNA in FFPE Tissue Sections

Source: O&#39;Sullivan, K. M. et al. Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis. J. Vis. Exp. (2020)
...

glomeruli isolation: a technique to obtain intact glomeruli from murine kidney

glomeruli-isolation-technique-to-obtain-intact-glomeruli-from-murine

Glomeruli Isolation: A Technique to Obtain Intact Glomeruli from Murine Kidney

Source: Eymael, J. et al. Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation. J. Vis. Exp. ...

murine renal cell isolation: a technique to isolate and generate primary culture of renal tubular epithelial cells

murine-renal-cell-isolation-technique-to-isolate-generate-primary

Murine Renal Cell Isolation: A Technique to Isolate and Generate Primary Culture of Renal Tubular Epithelial Cells

Source: Ding, W. et al. Isolation, Characterization and High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells. J. ...

periodic acid schiff or pas staining: a method to assess the structural deformities of glomerulus

periodic-acid-schiff-or-pas-staining-method-to-assess-structural

Periodic Acid Schiff or PAS Staining: A Method to Assess the Structural Deformities of Glomerulus

Source: Stevens, M., Oltean, S. Assessment of Kidney Function in Mouse Models of Glomerular Disease.&#160;J. Vis. Exp. (2018)
In this video, we describe ...

reverse phase protein arrays based protein expression analysis: a procedure for simultaneous quantification of expression of multiple proteins from cell lysate

reverse-phase-protein-arrays-based-protein-expression-analysis

Reverse Phase Protein Arrays Based Protein Expression Analysis: A Procedure for Simultaneous Quantification of Expression of Multiple Proteins from Cell Lysate

Source: O&#39;Mahony, F. C. et al. The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell ...

expansion pathology: a method for high-resolution optical imaging of clinical tissue samples

expansion-pathology-method-for-high-resolution-optical-imaging

Expansion Pathology: A Method for High-resolution Optical Imaging of Clinical Tissue Samples

Source: Klimas, A. et al. Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion.&#160;J. Vis. Exp.&#160;(2019)
In this video, ...

generating renal cell carcinoma model: a protocol to develop orthotopic rcc murine model by intrarenal implantation of cancer cells

generating-renal-cell-carcinoma-model-protocol-to-develop-orthotopic

Generating Renal Cell Carcinoma Model: A Protocol to Develop Orthotopic RCC Murine Model by Intrarenal Implantation of Cancer Cells

Source: Murphy, K. A. et al. A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical ...

bladder tumor organoid model: a method for orthotopic transplantation of bladder organoids into recipient murine bladder

bladder-tumor-organoid-model-method-for-orthotopic-transplantation

Bladder Tumor Organoid Model: A Method for Orthotopic Transplantation of Bladder Organoids into Recipient Murine Bladder

Source: Kim, Y. et al. Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids.&#160;J. Vis. Exp.&#160;(2020)
In this ...

intravenous microinjection of nephrotoxins into zebrafish embryo: a technique to deliver nephrotoxic agents into the bloodstream of zebrafish embryo

intravenous-microinjection-nephrotoxins-into-zebrafish-embryo

Intravenous Microinjection of Nephrotoxins into Zebrafish Embryo: A Technique to Deliver Nephrotoxic Agents into the Bloodstream of Zebrafish Embryo

Source: McKee, Robert A. et al. Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury. J. Vis. Exp.&#160;(2016)
This video demonstrates the ...

patient-derived tumor xenografting: a technique to generate experimental mouse model for evaluating urothelial cell carcinoma

patient-derived-tumor-xenografting-technique-to-generate-experimental

Patient-derived Tumor Xenografting: A Technique to Generate Experimental Mouse Model for Evaluating Urothelial Cell Carcinoma

Source: Moret, R. et al. Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and ...

modeling orthotopic bladder tumor in mouse model: a procedure for intravesical administration of cancer cells into murine bladder

modeling-orthotopic-bladder-tumor-mouse-model-procedure-for

Modeling Orthotopic Bladder Tumor in Mouse Model: A Procedure for Intravesical Administration of Cancer Cells into Murine Bladder

Source: Tham, S. M. et al. A Murine Orthotopic Bladder Tumor Model and Tumor Detection System.&#160;J. Vis. Exp.&#160;(2017)
In this video, we describe ...

unilateral ureteral obstruction murine model: a surgical procedure to obstruct the urinary flow from a kidney in mouse models

unilateral-ureteral-obstruction-murine-model-surgical-procedure-to

Unilateral Ureteral Obstruction Murine Model: A Surgical Procedure to Obstruct the Urinary Flow from a Kidney in Mouse Models

Source: Yanai, K. et al. Quantitative real-time PCR evaluation of microRNA expressions in mouse kidney with unilateral ureteral obstruction. J. Vis. Exp. ...

urine depletion and bladder rinsing in mouse model: a catheter-based procedure to empty and wash murine urinary bladder

urine-depletion-bladder-rinsing-mouse-model-catheter-based-procedure

Urine Depletion and Bladder Rinsing in Mouse Model: A Catheter-based Procedure to Empty and Wash Murine Urinary Bladder

Source: Yu, C. et al. Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation. J. Vis. Exp. (2018)
In this video, we describe ...

delivery of dna plasmids into urothelium of mouse bladder: an electroporation-based procedure to efficiently deliver dna plasmids into urothelial cells of murine bladder to generate genetically engineered models

delivery-dna-plasmids-into-urothelium-mouse-bladder-an

Delivery of DNA Plasmids into Urothelium of Mouse Bladder: An Electroporation-based Procedure to Efficiently Deliver DNA Plasmids into Urothelial Cells of Murine Bladder to Generate Genetically Engineered Models

Source: Chuan, Y. et al. Novel method of plasmid DNA delivery to mouse bladder urothelium by electroporation. J. Vis. Exp.&#160; (2018)
This video ...

hydrodynamic renal pelvis injection: a technique to directly deliver dna into kidney cells of murine model

hydrodynamic-renal-pelvis-injection-technique-to-directly-deliver-dna

Hydrodynamic Renal Pelvis Injection: A Technique to Directly Deliver DNA into Kidney Cells of Murine Model

Source: Woodard, L. E. et al. Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney. J. Vis. Exp. (2018)
In this video, ...

intrarenal drug delivery in rats: a method to deliver therapeutic drugs in rat kidney via intra-arterial injection

intrarenal-drug-delivery-rats-method-to-deliver-therapeutic-drugs-rat

Intrarenal Drug Delivery in Rats: A Method to Deliver Therapeutic Drugs in Rat Kidney via Intra-arterial Injection

Source: Franchi, et al. Intravascular Delivery of Biologics to the Rat Kidney. J. Vis. Exp.&#160; (2016)
In this video, we demonstrate intra-renal drug ...

fluid aspiration from mouse glomeruli: a method to aspirate glomerular fluid from mouse using two-photon mediated micropuncture

fluid-aspiration-from-mouse-glomeruli-method-to-aspirate-glomerular

Fluid Aspiration from Mouse Glomeruli: A Method to Aspirate Glomerular Fluid from Mouse Using Two-Photon Mediated Micropuncture

Source: Matsushita, K. et al. Micropuncture of Bowman&#39;s Space in Mice Facilitated by 2 Photon Microscopy. J. Vis. Exp. (2018)
This video showcases ...

preparation of decellularized rat kidney: a procedure to generate acellular vascularized whole-organ scaffold from a harvested rat kidney

preparation-decellularized-rat-kidney-procedure-to-generate-acellular

Preparation of Decellularized Rat Kidney: A Procedure to Generate Acellular Vascularized Whole-organ Scaffold from a Harvested Rat Kidney

Source: Kim, E. H. et al. Preparation of Decellularized Kidney Scaffolds in Rats. J. Vis. Exp. (2021)
This video describes the preparation of a ...

extracting intact kidney from mouse: a technique to obtain kidney without renal capsule from murine models

extracting-intact-kidney-from-mouse-technique-to-obtain-kidney

Extracting Intact Kidney from Mouse: A Technique to Obtain Kidney Without Renal Capsule from Murine Models

depigmentation and prestaining of zebrafish kidney: a technique to prepare adult zebrafish kidney for staining

depigmentation-prestaining-zebrafish-kidney-technique-to-prepare

Depigmentation and Prestaining of Zebrafish Kidney: A Technique to Prepare Adult Zebrafish Kidney for Staining

Source: McCampbell, K. K. et al. Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney. J. Vis. Exp. (2014)
In this video, we ...

kidney flat mount preparation: a procedure to dissect and prepare a flat mount for microscopic analysis of adult zebrafish kidney

kidney-flat-mount-preparation-procedure-to-dissect-prepare-flat-mount

Kidney Flat Mount Preparation: A Procedure to Dissect and Prepare a Flat Mount for Microscopic Analysis of Adult Zebrafish Kidney

Source: McCampbell, K. K. et al. Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney.&#160;J. Vis. Exp. (2014)
This video ...

renal organotypic culture on chicken cam: a technique for transplantation of murine kidney explants on chicken cam to induce formation of renal vascular architecture

renal-organotypic-culture-on-chicken-cam-technique-for

Renal Organotypic Culture on Chicken CAM: A Technique for Transplantation of Murine Kidney Explants on Chicken CAM to Induce Formation of Renal Vascular Architecture

Source: Kaisto, S. et al. Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy ...

Encyclopedia Of Experiments - cancer research