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第 32 章

細胞とタンパク質の分析

細胞の分離と単離の概要
細胞の分離と単離の概要
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, ...
細胞培養
細胞培養
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are ...
細胞株
細胞株
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number ...
ハイブリドーマテクノロジー
ハイブリドーマテクノロジー
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or ...
組織の均質化と細胞溶解
組織の均質化と細胞溶解
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. ...
細胞内分画
細胞内分画
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular ...
フローサイトメトリー
フローサイトメトリー
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing ...
カラムクロマトグラフィーの原理
カラムクロマトグラフィーの原理
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. ...
カラムクロマトグラフィーの種類
カラムクロマトグラフィーの種類
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating ...
免疫沈降
免疫沈降
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native ...
タグ付けと融合タンパク質
タグ付けと融合タンパク質
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from ...
SDSページ
SDSページ
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under ...
ウェスタンブロッティング
ウェスタンブロッティング
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting ...
二次元ゲル電気泳動
二次元ゲル電気泳動
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method ...
酵素結合免疫吸着アッセイ
酵素結合免疫吸着アッセイ
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays ...
MALDI-TOF質量分析
MALDI-TOF質量分析
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological ...
タンデム質量分析を用いたペプチド同定
タンデム質量分析を用いたペプチド同定
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass ...
生体試料のX線回折
生体試料のX線回折
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, ...
生物学におけるNMRの応用
生物学におけるNMRの応用
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an ...
プロテオミクス
プロテオミクス
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, ...
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