Electroporation of Agrobacterium tumefaciens for Genetic Engineering of Microalgal Cells

Begin by adding 50 microliters of electrocompetent Agrobacterium tumefaciens cells into a pre-chilled 0.1-millimeter Q-vet. Add two microliters of pCAMBIA1302 to the Q-vet. now use an electroporator at 2, 400 volts to eelectroporate the cells.