Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (CPCR)

Begin by pipetting out 0.5 milliliters of a log phase culture of chlorella vulgaris. Spread the culture on at Tris-Acetate-Phosphate or TAP Agar plate. Grow the culture for five days at 25 degrees Celsius.

Next, inoculate 10 milliliters of s