Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells

To begin, prepare a 1.8%agarose plate with HEPES Buffered E3 medium or E3.While working under a dissection microscope, place six to 10 anesthetized larvae in a row on the agarose plate. Put one insect pin on the head of each larvae. Then remove th