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Maastricht University Medical Center

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Wendy L.J. Hansen¹, Judith Beuving¹, Annelies Verbon², Petra. F.G. Wolffs¹

¹Department of Medical Microbiology, Maastricht University Medical Center, ²Department of Internal Medicine, Erasmus Medical Center

The design of a straightforward one-day workflow scheme for bacterial pathogen diagnostics enables the rapid recognition of bloodstream infections. The inclusion of eight clinically relevant bacterial targets and their antibiotic resistance profiles offers the clinician an initial insight on the same day, which can lead to more adequate therapy.

Developmental Biology

Gut Isolation from Zebrafish Larvae for Single-cell RNA Sequencing

Naomi J. M. Kakiailatu¹, Laura E. Kuil^1,2, Eric Bindels³, Joke T. M. Zink³, Michael Vermeulen³, Veerle Melotte⁴, Maria M. Alves¹

¹Department of Clinical Genetics, Erasmus University Medical Center - Sophia Children's Hospital, ²Division of Psychosocial Research and Epidemiology, the Netherlands Cancer Institute, ³Department of Hematology, Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology, Maastricht University Medical Center

Here, we describe a method for gut isolation from zebrafish larvae at 5 days post fertilization, for single-cell RNA sequencing analysis.

Cancer Research

Radiosynthesis, Quality Control, and Small Animal Positron Emission Tomography Imaging of ⁶⁸Ga-Labelled Nano Molecules

Betül Altunay¹, Matthias Bauwens^1,2,3, Laura Schäfer¹, Amelie Heesch¹, Agnieszka Morgenroth¹, Felix M. Mottaghy^1,2,4, Alexandru Florea^1,2,4

¹Department of Nuclear Medicine, University Hospital RWTH Aachen, ²Department of Radiology and Nuclear Medicine, Maastricht University Medical Center, ³School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University, ⁴School for Cardiovascular Diseases (CARIM), Maastricht University

This paper describes the radiosynthesis, formulation, quality control of a new radiolabeled probe (i.e., ⁶⁸Ga-labeled nanobody NM-02), and its use for small animal PET/CT imaging in a xenograft model.