Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA

To begin, take EVs isolated from Calu6 cells, transfected with fluorophore-conjugated EV-micro RNA, and cell-micro RNA. Dilute the EVs in an appropriate volume of ultra-filtered PBS to prepare the extracellular vesicle suspension for flow cytometr