Optimization and In Vivo Propagation of CellTrace Far Red (CT-FR) Stained Zebrafish T-ALL Cells

To begin, collect one time 10 to the power of six fluorescently labeled zebrafish, T-ALL cells in 1.5 milliliter microcentrifuge tubes. Centrifuge the cells at 2, 500 G for five minutes at four degrees Celsius and resuspend the pellet in one milli