Scratch Wound Migration Assay of Human Umbilical Vein Endothelial Cells (HUVECs) for Investigating Angiogenesis In Vitro

To begin, seed 100 microliters of HUVEC cells into the wells of a 96 well plate. Place the plate in a cell culture incubator at 37 degrees Celsius under 5%carbon dioxide for six hours to facilitate cell settling. Next, replace the medium in each w