Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay
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09:16 min
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May 31st, 2024
DOI :
May 31st, 2024
•Julian Rapp*1,2, Jan Ness*1,3, Paula Liang1, Martin J. Hug4, Hansjürgen Agostini1, Günther Schlunck1, Clemens Lange5, Felicitas Bucher1
1Eye Center, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 2Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 3Institute of Pharmaceutical Sciences, Faculty of Chemistry and Pharmacy, University of Freiburg, 4Pharmacy, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 5Ophtha-Lab, Department of Ophthalmology, St. Franziskus Hospital Muenster
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Chapters in this video
0:00
Introduction
1:30
Scratch Wound Migration Assay of Human Umbilical Vein Endothelial Cells (HUVECs) for Investigating Angiogenesis In Vitro
3:13
Fixing and Blocking 2D Human Umbilical Vein Endothelial Cell (HUVEC) Cultures for Immunohistochemical Analysis
4:49
Spheroid Sprouting Assay of Human Umbilical Vein Endothelial Cells (HUVECs) for Investigating Angiogenesis In Vitro
7:41
Immunocytochemistry of Human Umbilical Vein Endothelial Cell (HUVEC) Spheroids in a 3D Collagen Matrix for Investigating Angiogenesis In Vitro
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