Spheroid Sprouting Assay of Human Umbilical Vein Endothelial Cells (HUVECs) for Investigating Angiogenesis In Vitro

To begin, add HUVECs into a 10 milliliter Falcon tube containing eight milliliters of endothelial growth medium. Add two milliliters of METHOCEL stock solution into the tube and shake well. Next, transfer 25 microliters of the mixture onto the inv