Preparation of Hyaluronic Acid-Collagen Hydrogel in a Tissue Culture Insert for Replicating the Glioma Tumor Microenvironment

To begin, place collagen, sodium hydroxide, sterile ultrapure water, 10 times PBS, one micro centrifuge tube, methacrylated hyaluronic acid, and the photo initiator on ice. Then place the tissue culture inserts in the plate and label it. To prepar