Nucleosome Reconstitution for Single-Molecule Chromatin Studies

To begin, add 500 microliters of protein-free image buffer to channel 3 of the flow cell. Mix two nanomolar of Saccharomyces cerevisiae NAP1 and two to five nanomolar of LD655-labeled H4 histone octamer with 500 microliters of 1X HR buffer. Add th