the accelerating rotating rod assay or rotarod test: a method to test motor coordination and learning in mice

The Accelerating Rotating Rod Assay or Rotarod Test: A Method to Test Motor Coordination and Learning in Mice

Acclimate the subjects to their lane reservoir for 10 minutes prior to the start of the trial. During this acclimation period, prepare the rotor rod apparatus, cleaning each lane of the apparatus with 30% ethanol.
Next, open the rotor rod software. Set the start settings to 4 rpm, acceleration settings to 5 minutes, and final speed settings to 40 rpm to provide a constant acceleration from 4 to 40 rpm over a period of 5 minutes.
After acclimation period, place the subjects onto the rotarod in their respective lanes while the rod is rotating at 4 rpm. Once all the subjects are in their respective lanes, begin the first trial. Record the latency to fall using the instrument software. A secondary timer can be employed as a backup. Following the trial completion, allow each subject to rest in their respective lane reservoirs for 10 minutes prior to the next trial to prevent fatigue.

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All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Accelerating Rotarod
<ol>
	<li>Acclimate subjects to the assay room 10 min prior to the start of the trial. During this acclimation period, prepare the rotarod apparatus, cleaning each lane of the apparatus with 30% ethanol.</li>
	<li>Open the rotarod software and set start settings to 4 rpm, acceleration settings to 5 min, and final speed settings to 40 rpm. These settings provide a constant acceleration from 4&#8211;40 rpm over a period of 5 min. Mice can remain on the rotarod at 40 rpm for an additional 5 min beyond the acceleration period for a maximum run length of 10 min.</li>
	<li>Post-acclimation, place subjects onto the rotarod in their respective lanes while the rod is rotating at 4 rpm. Once all subjects are in their respective lanes, begin the first trial. Record the latency to fall using the instrument software; a secondary timer can be employed as a backup.</li>
	<li>Following trial completion, allow each subject to rest in their respective lane reservoirs for ten minutes prior to the next trial to prevent fatigue.</li>
	<li>Repeat steps 1.3 and 1.4 for a total of four trials.</li>
	<li>Repeat steps 1.3&#8211;1.5 for a total of four consecutive days, continuing treatment throughout the trial period. Upon completion, quantify latency to fall off the rotating rod for each trial per subject.</li>
</ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>ANIMALS</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Wildtype mice</td>
			<td>The Jackson Laboratory</td>
			<td>1800</td>
			<td></td>
		</tr>
		<tr>
			<td>EQUIPMENT</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Rotallion Rotarod</td>
			<td>PPP&#38;G</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>SOFTWARE</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>3P&#38;G Rota-Rod v3.3.3 (rotarod software)</td>
			<td>PPP&#38;G</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

Source: Ferro, A., et al. <a href="https://www.jove.com/video/53758/treating-sca1-mice-with-water-soluble-compounds-to-non-specifically">Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function</a>.&#160;J. Vis. Exp.&#160; (2017).
In this video, we perform the accelerating rotating rod or rotarod test to assess motor coordination and learning in mouse subjects.&#160;&#160;

Source: Ferro, A., et al. Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function.&#160;J. Vis. Exp.&#160; ...

The accelerating rotarod device, as the name suggests, consists of a rotating rod whose speed of rotation accelerates at a predetermined rate over a set time period. The rod is placed high enough to discourage subjects from jumping off, while dividers separate it into equally spaced lanes, keeping animals apart.
To perform the test, place the mice on the rotating rod in their respective lanes and then gradually increase the speed. Mice will grip tightly onto the rod to keep their balance and avoid falling off. Record the time until they fall across repeated trials to assess motor coordination and learning.

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The Accelerating Rotating Rod Assay or Rotarod Test: A Method to Test Motor Coordination and Learning in Mice

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