クロマチンの相互作用のマッピングと転写調節を理解するためのペアエンドタグシーケンシング（CHIA-PET）によるクロマチンの相互作用の解析

1:00

Overall View of Chromatin Interaction Analysis using Paired-end Tag (ChIA-PET)

2:15

ChIP - Chromatin Immunoprecipitation

4:24

Cell Lysis

5:07

Nuclear Lysis

7:11

Fragmentation of Chromatin

8:26

Washing, Preclearing, and Coating of Antibody to Beads

10:08

Chromatin Immunopreciptation

11:20

Washing and Elution

11:48

ChIA-PET - Chromatin Interaction Analysis using Paired-end Tag Sequencing

12:29

End-blunting of ChIP DNA Fragments

13:33

Ligation of Biotinylated Half-linkers to ChIP DNA

16:02

Elution and Proximity Ligation of ChIP DNA Fragments

18:08

Immobilization of ChIA-PET DNA to Streptavidin Beads

20:47

Conclusion