<h2 class="jove_title"><a href="https://www.jove.com/video/50525">Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy</a></h2>
Lisa Gfrerer1, Max Dougherty1, Eric C. Liao1
1<a href='/institutions/NA-north-america/US-united-states/MA-massachusetts/24866-massachusetts-general-hospital'>Center for Regenerative Medicine, Massachusetts General Hospital</a>
Visualization of experimental data has become a key element in presenting results to the scientific community. Generation of live time-lapse recording of growing embryos contributes to better presentation and understanding of complex developmental processes. This protocol is a step-by-step guide to cell labeling via photoconversion of kaede protein in zebrafish.
<h2 class="jove_title"><a href="https://www.jove.com/video/50582">Operation of a Benchtop Bioreactor</a></h2>
Kristina M. Obom1, Andrew Magno2, Patrick J. Cummings1
1Center for Biotechnology Education, Krieger School of Arts and Sciences, Johns Hopkins University, 2ATR Biotech
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture's biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
<h2 class="jove_title"><a href="https://www.jove.com/video/50604">Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion</a></h2>
Grady E. Carlson1, Eric W. Martin2, Monica M. Burdick1,2
1Department of Chemical and Biomolecular Engineering, Russ College of Engineering and Technology, Ohio University, 2Biomedical Engineering Program, Ohio University
Dual camera emission splitting systems for two-color fluorescence microscopy generate real-time image sequences with exceptional optical and temporal resolution, a requirement of certain live cell assays including parallel plate flow chamber adhesion assays. When software is employed to merge images from simultaneously acquired emission channels, pseudocolored image sequences are produced.
<h2 class="jove_title"><a href="https://www.jove.com/video/50757">Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry</a></h2>
Evan L. Pannkuk1, Thomas S. Risch2, Brett J. Savary3
1Graduate Program of Environmental Science, Arkansas State University, 2Department of Biological Sciences, Arkansas State University, 3Arkansas Biosciences Institute and College of Agriculture and Technology, Arkansas State University
Mammalian integument contains solvent-extractable lipids that can provide chemical compositions characteristic of individual species. This paper presents a routine method for separating broad lipid classes isolated from integumentary tissues using thin layer chromatography and determining the triacylglyceride profile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

september 2013 - this month in jove

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/79">September 2013 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
Palatogenesis (palate formation) involves complex developmental processes that are highly regulated and conserved among vertebrates. Thus, scientists use laboratory models like the zebrafish to study cleft palate and related developmental malformations. <a href="http://www.jove.com/video/50525">Gfrerer et al.</a> examine zebrafish embryos using time-lapse confocal microscopy, which lets them observe the migration of cranial neural crest cells (CNCCs) from the dorsal neural tube to form craniofacial structures. This method also allows live imaging of abnormal craniofacial development in zebrafish, which is used to model conditions like cleft palate in humans.
In the laboratory, bacteria, yeast, and many other cell types are commonly grown in shake flasks. However, this classic technique has many drawbacks that can prevent optimal growth, such as fluctuations in pH, oxygen concentration, nutrients, and temperature. In <a href="http://www.jove.com/bioengineering">JoVE Bioengineering</a>, <a href="http://www.jove.com/video/50582">Obom et al.</a> demonstrate a benchtop bioreactor complete with a pH probe an oxygen probe, reagent feed bottle to replenish media, precise temperature controls, a gas infuser, and an impeller that stirs the culture. This system allows optimal culture conditions for the large-scale growth of recombinant expression systems.
Also in <a href="http://www.jove.com/bioengineering">JoVE Bioengineering</a>, we have a method for live cell imaging in a flow-based cell adhesion assay. But unlike standard protocols that use single camera systems, our featured method is a dual camera emission splitting system that captures real-time images in two fluorescence channels at the same time. <a href="http://www.jove.com/video/50604">Carlson et al.</a> use a parallel plate flow chamber adhesion assay to demonstrate this dual camera system, which they use to study adhesion, rolling, and other cell behaviors.
<a href="http://www.jove.com/chem">JoVE Chemistry</a> features a not-so-common animal model: the Eastern red bat, which is widespread in the United States; however, in recent years, a fungal disease called white nose syndrome (WNS) has killed millions of these bats. WNS is a major threat to the agricultural industry, which relies on bats as a form of natural pest control. Like other mammals, bats have sebaceous glands in their skin that secrete sebum, an oily material that helps protect against infections. <a href="http://www.jove.com/video/50757">Pannkuk et al.</a> demonstrate a method for isolating the sebum from the hair and the skin of the bat, then analyze the lipid composition of the sebum using thin-layer chromatography (TLC) combined with matrix-assisted laser desorption/ionization time-of-flight (MALTI-TOF) mass spectrometry. The lipid profile of the sebum can affect immune processes and can also serve as disease markers; thus, this method will yield vital information for disease treatment and prevention in the Eastern red bat as well as other mammals.
You&#8217;ve just had a sneak peek of a few highlights from the <a href="http://www.jove.com/archive/79">September 2013 issue</a> of JoVE. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about September 2013 - This Month in JoVE at JoVE.com

September 2013 - This Month in JoVE

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/79">September 2013 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Here's a look at what's coming up in the September 2013 issue of JoVE: The Journal of Visualized Experiments.
Palatogenesis (palate formation) involves ...

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2.5K ビュー.  Massachusetts Eye and Ear. Here's a look at what's coming up in the September 2013 issue of JoVE: The Journal of Visualized Experiments.

ビデオ: September 2013 - This Month in JoVE

intraoperative assessment of resection margins in oral cavity cancer: this is the way

Intraoperative Assessment of Resection Margins in Oral Cavity Cancer: This is the Way

The goal of this protocol is to provide a clear overview of specimen-driven intraoperative assessment of resection margins. It is encouraged to implement this protocol to improve patient care at other...

measuring composition of cd95 death-inducing signaling complex and processing of procaspase-8 in this complex

Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex

Here, an experimental workflow is presented that enables the detection of caspase-8 processing directly at the death-inducing signaling complex (DISC) and determines the composition of this complex. This methodology has broad applications, from unraveling the molecular mechanisms of cell death pathways to the dynamic modeling of apoptosis...

experience is instrumental in tuning a link between language and cognition: evidence from 6-  to 7- month-old infants' object categorization

Experience is Instrumental in Tuning a Link Between Language and Cognition: Evidence from 6-  to 7- Month-Old Infants' Object Categorization

At 3-4 months, listening to human and nonhuman primate vocalizations boosts infants&#39; cognition; by 6 months, only human vocalizations exert this cognitive advantage. We describe an exposure manipulation that reveals the powerful shaping role of experience as infants specify which sounds to link to cognition and which to tune...

cox-maze iv procedure concomitant with valvular surgery in situs inversus dextrocardia: a single-center experience in china

Cox-Maze IV Procedure Concomitant with Valvular Surgery In Situs Inversus Dextrocardia: A Single-Center Experience in China

We summarize the Cox-Maze IV procedure concomitant with valvular surgery performed in patients with situs inversus dextrocardia at this...

in vivo assessment of microtubule dynamics and orientation in caenorhabditis elegans neurons

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

A protocol for imaging the dynamic microtubules in vivo using fluorescently labeled End binding protein has been presented. We described the methods to label, image, and analyze the dynamic microtubules in the Posterior lateral microtubule (PLM) neuron of C.

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

simulating temperature in a soil incubation experiment

Simulating Temperature in a Soil Incubation Experiment

Laboratory soil warming experiments usually employ two or more constant temperatures in multiple chambers. By presenting a sophisticated environmental chamber, we provide an accurate temperature control method to imitate the magnitude and amplitude of in situ soil temperature and improve the experimental design of soil incubation...

in vivo chronic two-photon imaging of microglia in the mouse hippocampus

In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

This paper describes a method for chronic in vivo observation of the resting microglia in the mouse hippocampal CA1 using precisely controlled surgery and two-photon...

In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

noninvasive intratracheal lipopolysaccharide instillation in mice

Noninvasive Intratracheal Lipopolysaccharide Instillation in Mice

Here, we propose a protocol for intratracheal lipopolysaccharide (LPS) delivery via noninvasive oropharyngeal endotracheal intubation. This method minimizes the trauma of the surgical procedure for the animal and accurately delivers LPS to the trachea and then to the lungs.

adjunctive diode laser therapy and probiotic lactobacillus therapy in the treatment of periodontitis and peri-implant disease

Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease

This article describes two protocols: 1) adjunctive diode laser therapy for treating periodontitis and 2) probiotic Lactobacillus therapy for treating peri-implant disease, with emphasis on the laser usage mode (inside or outside pocket), laser application regimen (single or multiple sessions), and a probiotic protocol of professional and home...

Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease

implantation of a cranial window for repeated in vivo imaging in awake mice

Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice

Presented here is a protocol for the implantation of a chronic cranial window for the longitudinal imaging of brain cells in awake, head-restrained...

Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice

intravitreal injections in the ovine eye

Intravitreal Injections in the Ovine Eye

Intravitreal injections were performed in the sheep eye with the aim of delivering viral-mediated gene therapy to the...

in vivo targeting of neural progenitor cells in ferret neocortex by in utero electroporation

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

Presented here is a protocol to perform genetic manipulation in the embryonic ferret brain using in utero electroporation. This method allows for targeting of neural progenitor cells in the neocortex in...

assaying for inorganic polyphosphate in bacteria

Assaying for Inorganic Polyphosphate in Bacteria

We describe a simple method for rapid quantification of inorganic polyphosphate in diverse bacteria, including Gram-negative, Gram-positive, and mycobacterial...

data acquisition and analysis in brainstem evoked response audiometry in mice

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Brainstem evoked response audiometry is an important tool in clinical neurophysiology. Nowadays, brainstem evoked response audiometry is also applied in the basic science and preclinical studies involving both pharmacological and genetic animal models. Here we provide a detailed description of how auditory brainstem responses can be successfully recorded and analyzed in...

nasal potential difference to quantify trans-epithelial ion transport in mice

Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice

Here, we present a protocol to measure nasal potential difference in mice. The test quantifies the function of transmembrane ion transporters such as the cystic fibrosis transmembrane conductance regulator and the epithelial sodium channel. It is valuable to evaluate the efficacy of novel therapies for cystic...

post-myocardial infarction heart failure in closed-chest coronary occlusion/reperfusion model in g&#246;ttingen minipigs and landrace pigs

Post-Myocardial Infarction Heart Failure in Closed-chest Coronary Occlusion/Reperfusion Model in G&amp;#246;ttingen Minipigs and Landrace Pigs

The overall goal of the current study is to present the techniques of induction of myocardial infarction (MI) and post-myocardial infarction heart failure (post-MI HF) in closed-chest, adult G&#246;ttingen minipigs and the characterization of post-MI HF model in G&#246;ttingen minipigs as compared to Landrace pigs.

Post-Myocardial Infarction Heart Failure in Closed-chest Coronary Occlusion/Reperfusion Model in G&#246;ttingen Minipigs and Landrace Pigs

preparation of gushukang (gsk) granules for in vivo and in vitro experiments

Preparation Of Gushukang GSK Granules for In Vivo and In Vitro Experiments

This article provides a detailed protocol for preparing a working solution of Gushukang granules for animal studies and GSK granule containing serum for in vitro experiments. This protocol can be applied to pharmacological investigations of herbal medicines as well as prescriptions for both in vivo and in vitro...

Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments

conducting respiratory oscillometry in an outpatient setting

Conducting Respiratory Oscillometry in an Outpatient Setting

We demonstrate a standard operating protocol to conduct respiratory oscillometry, highlighting key quality control and assurance procedures.

precise brain mapping to perform repetitive in vivo imaging of neuro-immune dynamics in mice

Precise Brain Mapping to Perform Repetitive In Vivo Imaging of Neuro-Immune Dynamics in Mice

This protocol describes a chronic cranial window implantation technique that can be used for longitudinal imaging of neuro-glio-vascular structures, interactions, and function in both healthy and diseased conditions. It serves as a complementary alternative to the transcranial imaging approach that, while often preferred, possesses some critical...

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...