<a href="https://www.jove.com/video/51704">Using Insect Electroantennogram Sensors on Autonomous Robots for Olfactory Searches</a>
Martinez, D., Arhidi, L., Demondion, E., Masson, J. B., Lucas, P.
UMR 7503, Laboratoire Lorrain de Recherche en Informatique et ses Applications (LORIA), Centre National de la Recherche Scientifique (CNRS), UMR 1392 iEES-Paris, Institut d'Ecologie et des Sciences de l'Environnement de Paris, Physics of Biological Systems, Institut Pasteur
We describe a protocol for using insect antennae in the form of electroantennograms (EAGs) on autonomous robots. Our experimental design allows stable recordings within a day and resolves individual odor patches up to 10 Hz. The efficiency of EAG sensors for olfactory searches is demonstrated in driving a robot toward an odor source.
<a href="https://www.jove.com/video/51846">Assessment of Murine Exercise Endurance Without the Use of a Shock Grid: An Alternative to Forced Exercise</a>
Conner, J. D., Wolden-Hanson, T., Quinn, L. S.
Research Service, VA Puget Sound Health Care System, Seattle Institute for Biomedical and Clinical Research, Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Washington, Geriatric Research, Education, and Clinical Center, VA Puget Sound Health Care System
A method to assess exercise endurance in laboratory mice without the use of a shock grid is demonstrated. This method is a humane refinement that can decrease the confounding effects of stress on experimental parameters.
<a href="https://www.jove.com/video/51540">Design and Construction of an Urban Runoff Research Facility</a>
Wherley, B. G., White, R. H., McInnes, K. J., Fontanier, C. H., Thomas, J. C., Aitkenhead-Peterson, J. A., et al. 
Soil and Crop Sciences Department, Texas A#&amp;M University, The Scotts Miracle-Gro Company
This paper describes the design, construction, and function of a 1,000 m2 facility containing 24 individual 33.6 m2 field plots equipped for measuring total runoff volumes with time and collection of runoff subsamples at selected intervals for quantification of chemical constituents in the runoff water from simulated home lawns.
<a href="https://www.jove.com/video/51669">In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy</a>
Malide, D., Me'tais, J. Y., Dunbar, C. E.
Light Microscopy Core Facility, NHLBI/NIH, Hematology Branch, NHLBI/NIH
Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.

august 2014: this month in jove - chemical sensing robots, tracking blood cell development, rodents on the treadmill, and simulating urban runoff

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/90/august-2014">August 2014 issue</a> of <a href="http://www.jove.com">JoVE: The Journal of Visualized Experiments</a>. 
The ability to detect scent is the most sensitive of all animal senses. Insects have highly sensitive olfactory systems, and rely on odor perception for many daily activities. Certain species (e.g., moths) can find their mates over hundreds of meters using olfaction. Like dogs, insects can be trained to sniff out explosives, illegal drugs, and even diseases in humans and plants.
In insects, olfactory perception starts in the antennae, and this month in <a href="http://www.jove.com/neuro">JoVE Neuroscience</a>, <a href="http://www.jove.com/video/51704">Martinez et al</a>. combine the powers of moth antennae with robots to create olfactory searching machines. This whole-insect setup can provide a day's worth of stable recordings, and can distinguish individual odor pulses much better than artificial chemical sensors. This robotic platform may not only advance our knowledge of olfactory coding, but may also improve strategies for automated olfactory searches.
In <a href="http://www.jove.com/biology">JoVE Biology</a>, we examine the production of blood cells, a process called hematopoiesis. All the different blood cells are derived from hematopoietic stem and progenitor cells (HSPCs). <a href="http://www.jove.com/vidoe/51669">Malide et al</a>. use 5 different fluorescent markers, delivered by lentiviral vectors, to label different cells. Using confocal/two-photon hybrid microscopy, they can then noninvasively track the cells as they undergo hematopoiesis in vivo. This produces high-resolution 3D images for studying tissue regeneration and other applications in regenerative medicine.
In some behavioral studies, electric footshock is used to study fear conditioning in mice. It is also used in exercise studies to force the mice to run. But it's possible that the shock-induced stress can confound the results. This month in <a href="http://www.jove.com/behavior">JoVE Behavior</a>, <a href="http://www.jove.com/51846">Connor et al</a>. demonstrate a non-invasive method of studying exercise endurance in mice. They allow the mice to become familiar with the treadmill apparatus, and use gentle tapping to encourage sedentary mice to keep running. Soon the mice will voluntarily run by themselves. This procedure takes the stress and fear out of experimental exercise for mice. Who knows - maybe this can be applied to sedentary humans some day.
In <a href="http://www.jove.com/enviro">JoVE Environment</a>, a major concern with the increasing urban landscape is poor irrigation of runoff water, which can release unwanted chemicals and sediments into the environment. This month, <a href="http://www.jove.com/video/51540">Wherley et al</a>. design and construct a 1000m2 landscape facility with individual plots of grass that simulate home lawns. An irrigation system allows runoff samples to be collected and analyzed for volume and chemical composition. Facilities like this can help guide best practices to minimize the environmental impact of urban living.
You've just had a sneak peek of the <a href="http://www.jove.com/archive/90/august-2014">August 2014 issue of JoVE</a>. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about August 2014: This Month in JoVE - Chemical Sensing Robots, Tracking Blood Cell Development, Rodents on the Treadmill, and Simulating Urban Runoff at JoVE.com

August 2014: This Month in JoVE - Chemical Sensing Robots, Tracking Blood Cell Development, Rodents on the Treadmill, and Simulating Urban Runoff

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/90">August 2014</a> issue of JoVE.

Here's a look at what's coming up in the August 2014 issue of JoVE: The Journal of Visualized Experiments. 
The ability to detect scent is the most ...

august-2014-this-month-jove-chemical-sensing-robots-tracking-blood

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1.9K ビュー.  Massachusetts Eye and Ear. Here's a look at what's coming up in the August 2014 issue of JoVE.

ビデオ: August 2014: This Month in JoVE - Chemical Sensing Robots, Tracking Blood Cell Development, Rodents on the Treadmill, and Simulating Urban Runoff

design and construction of an urban runoff research facility

Design and Construction of an Urban Runoff Research Facility

This paper describes the design, construction, and function of a 1,000 m2 facility containing 24 individual 33.6 m2 field plots equipped for measuring total runoff volumes with time and collection of runoff subsamples at selected intervals for quantification of chemical constituents in the runoff water from simulated home...

vegetated treatment systems for removing contaminants associated with surface water toxicity in agriculture and urban runoff

Vegetated Treatment Systems for Removing Contaminants Associated with Surface Water Toxicity in Agriculture and Urban Runoff

This article summarizes the design attributes and the effectiveness of treatment systems that treat urban stormwater and agriculture irrigation runoff to remove pesticides and other contaminants associated with aquatic...

designing and implementing nervous system simulations on lego robots

Designing and Implementing Nervous System Simulations on LEGO Robots

An approach to neural network modeling on the LEGO Mindstorms robotics platform is presented. The method provides a simulation tool for invertebrate neuroscience research in both the research lab and the classroom. This technique enables the investigation of biomimetic robot control...

a system for tracking the dynamics of social preference behavior in small rodents

A System for Tracking the Dynamics of Social Preference Behavior in Small Rodents

Described here is a novel automated experimental system that offers an alternative to the three-chamber test and also solves several caveats. This system supplies multiple behavioral parameters that enable rigorous analysis of small rodent behavioral dynamics during the social preference and social novelty preference...

cell tracking using photoconvertible proteins during zebrafish development

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate...

simulating imaging of large scale radio arrays on the lunar surface

Simulating Imaging of Large Scale Radio Arrays on the Lunar Surface

A simulation framework for testing the imaging capabilities of large-scale radio arrays on the lunar surface is presented. Major noise components are discussed, and a software pipeline is walked through with details on how to customize it for novel scientific...

evaluating the effect of roadside parking on a dual-direction urban street

Evaluating the Effect of Roadside Parking on a Dual-Direction Urban Street

In this study, the effect of roadside parking on an urban street is analyzed. The entire process consists of traffic data gathering, data processing, operation simulation, simulation calibration, and sensitivity...

chemical gardens as flow-through reactors simulating natural hydrothermal systems

Chemical Gardens as Flow-through Reactors Simulating Natural Hydrothermal Systems

We describe chemical garden formation via injection experiments that allow for laboratory simulations of natural chemical garden systems that form at submarine hydrothermal...

using insect electroantennogram sensors on autonomous robots for olfactory searches

Using Insect Electroantennogram Sensors on Autonomous Robots for Olfactory Searches

We describe a protocol for using insect antennae in the form of electroantennograms (EAGs) on autonomous robots. Our experimental design allows stable recordings within a day and resolves individual odor patches up to 10 Hz. The efficiency of EAG sensors for olfactory searches is demonstrated in driving a robot toward an odor...

short session high intensity interval training and treadmill assessment in aged mice

Short Session High Intensity Interval Training and Treadmill Assessment in Aged Mice

Short session (&#8804;10 min) high intensity interval training (HIIT) is emerging as an alternative to longer exercise modalities, yet the shorter variants are rarely modeled in animal studies. Here, we describe a 10 min, 3 day a week, uphill treadmill HIIT protocol that enhances physical performance in male and female aged...

simulating impacts of ice storms on forest ecosystems

Simulating Impacts of Ice Storms on Forest Ecosystems

Ice storms are important weather events that are challenging to study because of difficulties in predicting their occurrence. Here, we describe a novel method for simulating ice storms that involves spraying water over a forest canopy during sub-freezing...

simulating temperature in a soil incubation experiment

Simulating Temperature in a Soil Incubation Experiment

Laboratory soil warming experiments usually employ two or more constant temperatures in multiple chambers. By presenting a sophisticated environmental chamber, we provide an accurate temperature control method to imitate the magnitude and amplitude of in situ soil temperature and improve the experimental design of soil incubation...

free radicals in chemical biology: from chemical behavior to biomarker development

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Radical-based biomimetic chemistry has been applied to building-up libraries necessary for biomarker...

activity-based training on a treadmill with spinal cord injured wistar rats

Activity-based Training on a Treadmill with Spinal Cord Injured Wistar Rats

This protocol demonstrates our model of activity-based locomotor treadmill training for rats with spinal cord injury (SCI). Included is both quadrupedal and forelimb-only groups, in addition to two distinct types of non-trained control groups. Investigators are able to assess training effects on SCI rats using this...

the lower body positive pressure treadmill for knee osteoarthritis rehabilitation

The Lower Body Positive Pressure Treadmill for Knee Osteoarthritis Rehabilitation

Here, based on a clinician&#8217;s point-of-view, we propose a two-model lower body positive pressure (LBPP) protocol (walking and squatting models) in addition to a clinical, functional assessment methodology, including details for further encouragement of the development of non-drug surgical intervention strategies in knee osteoarthritis patients. However, we only present the effect of LBPP training in improvement of pain and knee function in one patient through three-dimensional gait...

real-time electrocardiogram monitoring during treadmill training in mice

Real-Time Electrocardiogram Monitoring During Treadmill Training in Mice

Electrocardiogram (ECG) is the key variable to understanding cardiac electrophysiology. Physical exercise has beneficial effects but may also be harmful in the context of cardiovascular diseases. This manuscript provides a method of recording real-time ECG during exercise, which can serve to investigate its effects on cardiac electrophysiology in...

exploring the potential of mesenchymal stem cell sheet on the development of hepatocellular carcinoma in vivo

Exploring the Potential of Mesenchymal Stem Cell Sheet on The Development of Hepatocellular Carcinoma In Vivo

Here, we present a protocol to develop an in vivo cancer model using cell sheet technology. Such a model could be very useful for the evaluation of anticancer...

Exploring the Potential of Mesenchymal Stem Cell Sheet on The Development of Hepatocellular Carcinoma In Vivo

combining eye-tracking data with an analysis of video content from free-viewing a video of a walk in an urban park environment

Combining Eye-tracking Data with an Analysis of Video Content from Free-viewing a Video of a Walk in an Urban Park Environment

The objective of the protocol is to detail how to collect video data for use in the laboratory; how to record eye-tracking data of participants looking at the data and how to efficiently analyze the content of the videos that they were looking at using a machine learning...

assembly and tracking of microbial community development within a microwell array platform

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

The development of microbial communities depends on a combination of factors, including environmental architecture, member abundance, traits, and interactions. This protocol describes a synthetic, microfabricated environment for the simultaneous tracking of thousands of communities contained in femtoliter wells, where key factors such as niche size and confinement can be...

bs3 chemical crosslinking assay: evaluating the effect of chronic stress on cell surface gabaa receptor presentation in the rodent brain

BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain

The BS3 chemical crosslinking assay reveals reduced cell surface GABAA receptor expression in mouse brains under chronic psychosocial stress...

BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...