Wheat Germ Agglutinin Staining and Matrix Visualization (Plate 2)
5:53
Staining Viable and Dead Cells for Fluorescent Imaging (Plate 3)
6:44
Green-to-Red Fluorescent Signal Quantification (Plate 4)
7:16
Results: Assay Platform Effective for Analysis on Three Endpoints
8:56
Conclusion
文字起こし
The, overall, goal of this assay platform is to provide a meaningful screening work flow combining three, relevant, end points, viability, biomass, and biofilm matrix, for identification and characterization of new, anti-biofilm compounds. This pl
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Biofilm infections show high tolerance towards chemotherapy. No single assay captures the complexity of biofilms. Instead, complementary assays are needed. We present a screening platform (developed for S. aureus) that combines assays for viability, biomass, and biofilm matrix. It allows anti-biofilm drug discovery, including the assessment of long-term chemotherapeutic effects.