Name: november 2014: this month in jove: flow cytometry in space, venom extraction from spiders, decision making in bumblebees, and oceanic microbial communities
Uploaded: 2014-11-05T15:30:00
Duration: 3 min 39 s
Description: Watch this Scientific Journal Video about November 2014: This Month in JoVE: Flow Cytometry in Space, Venom Extraction from Spiders, Decision Making in Bumblebees, and Oceanic Microbial Communities at

<h2 class="jove_title"><a href="https://www.jove.com/video/51743">Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology</a></h2>
William S. Phipps*1, Zhizhong Yin*1, Candice Bae1, Julia Z. Sharpe1, Andrew M. Bishara2, Emily S. Nelson3, Aaron S. Weaver3, Daniel Brown4, Terri L. McKay3, DeVon Griffin3, Eugene Y. Chan1
1DNA Medicine Institute, 2Harvard Medical School, 3NASA Glenn Research Center, 4ZIN Technologies
Spaceflight blood diagnostics need innovation. Few demonstrations have been published illustrating in-flight, reduced-gravity health diagnostic technology. Here we present a method for construction and operation of a parabolic flight test rig for a prototype point-of-care flow-cytometry design, with components and preparation strategies adaptable to other setups.
<h2 class="jove_title"><a href="https://www.jove.com/video/52033">Radio Frequency Identification and Motion-sensitive Video Efficiently Automate Recording of Unrewarded Choice Behavior by Bumblebees</a></h2>
Levente L. Orb&#225;n, Catherine M.S. Plowright
School of Psychology, University of Ottawa
This video describes Radio-Frequency Identification (RFID) and motion-sensitive video recording methods to monitor choice behavior by bumblebees. 
<h2 class="jove_title"><a href="https://www.jove.com/video/51618">Extraction of Venom and Venom Gland Microdissections from Spiders for Proteomic and Transcriptomic Analyses</a></h2>
Jessica E. Garb
Department of Biological Sciences, University of Massachusetts Lowell
This article provides a protocol for the extraction of venom from spiders using electrical stimulation in order to 1) conduct proteomic characterization, 2) stimulate venom gland gene expression, and 3) perform functional studies of venoms. This is followed by a description of venom gland microdissections for gene expression studies.
<h2 class="jove_title"><a href="https://www.jove.com/video/52131">Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology</a></h2>
Andreas Florian Haas1, Ben Knowles1, Yan Wei Lim1, Tracey McDole Somera2, Linda Wegley Kelly1, Mark Hatay1, Forest Rohwer1
1Department of Biology, San Diego State University, 2Scripps Institution of Oceanography, University of California San Diego
Here, we present a comprehensive protocol to assess the organic and inorganic nutrient availability and the abundance and structure of microbial and viral communities in remote marine environments.

november 2014: this month in jove: flow cytometry in space, venom extraction from spiders, decision making in bumblebees, and oceanic microbial communities

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/93">November 2014 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
In <a href="http://www.jove.com/biology">JoVE Biology</a>, we have a lot of videos that use <a href="http://www.jove.com/search?q=flow+cytometry">flow cytometry</a>, which detects particles in suspension based on their fluorescence or their ability to absorb or scatter light. This technology allows blood and other heterogeneous cell mixtures to be sorted into cell populations. This has many biomedical applications, and this month, JoVE takes flow cytometry to a whole new level with a protocol that can be performed in space.
<a href="http://www.jove.com/video/51743">Phipps et al.</a> demonstrate how to construct a flow cytometry rig that can operate in zero-gravity conditions. Then, they take it aboard a reduced-gravity aircraft, which creates a weightless environment at certain points in its parabolic flight path. Besides showing how to operate the flow cytometer, Phipps et al. demonstrate techniques for staying in control and avoiding nausea on reduced-gravity aircrafts, known affectionately as "vomit comets" to those who have flown on them. With these methods, vital diagnostics and experiments based on flow cytometry may be taken into space.
Also in <a href="http://www.jove.com/biology">JoVE Biology</a>, we examine the western black widow spider. The female western widow makes deadly venom that is full of neurotoxins, so scientists are interested in isolating and characterizing the venom's components. <a href="http://www.jove.com/video/51618">Garb</a> demonstrates how to extract the venom, which can be subjected to protein analysis or functional assays. It also shows how to isolate venom glands from the spiders for gene expression studies. These methods may reveal important cellular pathways that are induced by venom, or provide leads for drug development.
In <a href="http://www.jove.com/neuro">JoVE Neuroscience</a>, bees are often used to model learning, memory, and behavior. This month, <a href="http://www.jove.com/video/52033">Orb&#225;n and Plowright</a> use two automated systems to study choice behavior in bumblebees One method is based on radio frequency identification (RFID). The researchers place RFID-enabled tags on the bumblebees and show them artificial flowers with RFID readers. Every time a tagged bumblebee enters a flower, it is automatically recorded. In a second method, the bumblebees receive colored tags with numbers, and motion-sensitive high-definition video cameras capture various behaviors associated with choice, like approach, hovering, and landing. These techniques can reveal how bees choose one flower over another, and delve into the root of perception and choice in animals.
In <a href="http://www.jove.com/enviro">JoVE Environment</a>, many natural resources come from the ocean, and tiny microorganisms and chemicals are critical components of marine ecosystems. This month, <a href="http://www.jove.com/video/52131">Haas et al.</a> describe how to collect seawater samples, and present a series of validated protocols to analyze chemical and microbial components. The set of protocols provides an up to date approach to studying the microscopic and chemical makeup of the ocean.
You've just had a sneak peek of the <a href="http://www.jove.com/archive/93">November 2014 issue</a> of JoVE. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about November 2014: This Month in JoVE: Flow Cytometry in Space, Venom Extraction from Spiders, Decision Making in Bumblebees, and Oceanic Microbial Communities at

November 2014: This Month in JoVE: Flow Cytometry in Space, Venom Extraction from Spiders, Decision Making in Bumblebees, and Oceanic Microbial Communities

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/93">November 2014</a> issue of JoVE.

Here's a look at what's coming up in the November 2014 issue of JoVE: The Journal of Visualized Experiments.
In JoVE Biology, we have a lot of videos that ...

november-2014-this-month-jove-flow-cytometry-space-venom-extraction

Without Section

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...