<h2 class="jove_title"><a href="https://www.jove.com/video/52154">Fecal Microbiota Transplantation via Colonoscopy for Recurrent C. difficile Infection</a></h2>
Jessica R. Allegretti, Joshua R. Korzenik, Matthew J. Hamilton
Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women&#8216;s Hospital
Fecal Microbiota Transplantation via colonoscopy is a safe and effective treatment for recurrent and refractory C.difficile infection. A systematic approach to patient and donor screening, preparation of stool, and delivery of the stool during the colonoscopy will maximize therapeutic success.
<h2 class="jove_title"><a href="https://www.jove.com/video/51039">Conducting Miller-Urey Experiments</a></h2>
Eric T. Parker1, James H. Cleaves2,3, Aaron S. Burton4, Daniel P. Glavin5, Jason P. Dworkin5, Manshui Zhou1, Jeffrey L. Bada6, Facundo M. Fern&#225;ndez1
1School of Chemistry and Biochemistry, Georgia Institute of Technology, 2Earth-Life Science Institute, Tokyo Institute of Technology, 3Institute for Advanced Study, 4Astromaterials Research and Exploration Science Directorate, NASA Johnson Space Center, 5Goddard Center for Astrobiology, NASA Goddard Space Flight Center, 6Geosciences Research Division, Scripps Institution of Oceanography, University of California at San Diego
The Miller-Urey experiment was a pioneering study regarding the abiotic synthesis of organic compounds with possible relevance to the origins of life. Simple gases were introduced into a glass apparatus and subjected to an electric discharge, simulating the effects of lightning in the primordial Earth&#8217;s atmosphere-ocean system. The experiment was conducted for one week, after which, the samples collected from it were analyzed for the chemical building blocks of life.
<h2 class="jove_title"><a href="https://www.jove.com/video/50864">Methods for Studying the Mechanisms of Action of Antipsychotic Drugs in Caenorhabditis elegans</a></h2>
Limin Hao1,2, Edgar A. Buttner1,2
1Department of Psychiatry, Harvard Medical School, 2Mailman Research Center, McLean Hospital
Approaches for testing the effects of antipsychotic drugs (APDs) in Caenorhabditis elegans are demonstrated. Assays are described for testing drug effects on development and viability and on pharyngeal pumping rate. These methods are also applicable for pharmacogenetic experiments with drug classes other than APDs.
<h2 class="jove_title"><a href="https://www.jove.com/video/51056">Utility of Dissociated Intrinsic Hand Muscle Atrophy in the Diagnosis of Amyotrophic Lateral Sclerosis</a></h2>
Parvathi Menon1,2, Steve Vucic1,2
1Department of Neurology, Westmead Hospital, 2Sydney Medical School, University of Sydney, Australia
Dissociated atrophy of intrinsic hand muscles, termed the split hand, appears to be a specific feature of amyotrophic lateral sclerosis (ALS).&#160;Consequently, a novel neurodiagnostic test, termed the split hand&#160;index, was developed to quantify the clinical phenomenon of the split hand, which differentiated ALS from mimic disorders.
<h2 class="jove_title"><a href="https://www.jove.com/video/51234">Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun</a></h2>
Matthew D. Mattozzi1,2, Mathias J. Voges1,2,3, Pamela A. Silver1,2, Jeffrey C. Way1,2
1Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Systems Biology, Harvard Medical School, 3Department of Biotechnology, Delft University of Technology
This work describes a novel method for selectively targeting subcellular organelles in plants, assayed using the BioRad Gene Gun.
<h2 class="jove_title"><a href="https://www.jove.com/video/51476">Coordinate Mapping of Hyolaryngeal Mechanics in Swallowing</a></h2>
Thomas Z. Thompson1, Farres Obeidin1, Alisa A. Davidoff2, Cody L. Hightower1, Christohper Z. Johnson1, Sonya L. Rice1, Rebecca-Lyn Sokolove1, Brandon K. Taylor1, John M. Tuck1, William G. Pearson, Jr.3,4
1Medical College of Georgia, Georgia Regents University, 2Department of Communicative Sciences and Disorders, New York University, 3Department of Cellular Biology &amp; Anatomy, Georgia Regents University, 4Department of Otolaryngology, Georgia Regents University
Coordinate mapping is a method of documenting salient features of hyolaryngeal biomechanics in the pharyngeal phase of swallowing. This methodology uses image analysis software to record coordinates of anatomical landmarks. These coordinates are imported into an excel macro and translated into kinematic variables of interest useful in dysphagia research.
<h2 class="jove_title"><a href="https://www.jove.com/video/51541">Laboratory Drop Towers for the Experimental Simulation of Dust-aggregate Collisions in the Early Solar System</a></h2>
J&#252;rgen Blum, Eike Beitz, Mohtashim Bukhari, Bastian Gundlach, Jan-Hendrik Hagemann, Daniel Hei&#223;elmann, Stefan Kothe, Rainer Schr&#228;pler, Ingo von Borstel, Ren&#233; Weidling
Institut f&#252;r Geophysik und extraterrestrische Physik, Technische Universit&#228;t Braunschweig
We present a technique to achieve low-velocity to intermediate-velocity collisions between fragile dust aggregates in the laboratory. For this purpose, two vacuum drop-tower setups have been developed that allow collision velocities between &#60;0.01 and ~10 m/sec. The collision events are recorded by high-speed imaging.
<h2 class="jove_title"><a href="https://www.jove.com/video/51605">Behavioral Assessment of the Aging Mouse Vestibular System</a></h2>
Victoria W. K. Tung1, Thomas J. Burton2, Edward Dababneh1, Stephanie L. Quail1, Aaron J. Camp1
1Discipline of Biomedical Science, University of Sydney, 2The Bosch Institute Animal Behavioural Facility, University of Sydney
Motor control and balance performance are known to deteriorate with age. This paper presents a number of standard noninvasive behavioral tests with the addition of a simple rotary stimulus to challenge the vestibular system and show changes in balance performance in a murine model of aging.
<h2 class="jove_title"><a href="https://www.jove.com/video/51669">In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy</a></h2>
Daniela Malide1, Jean-Yves M&#233;tais2, Cynthia E. Dunbar2
1Light Microscopy Core Facility, NHLBI/NIH, 2Hematology Branch, NHLBI/NIH
Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation. 
<h2 class="jove_title"><a href="https://www.jove.com/video/51826">Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration</a></h2>
&#205;lida Ortega1, Farshid Sefat1, Pallavi Deshpande1, Thomas Paterson1, Charanya Ramachandran3, Anthony J. Ryan2, Sheila MacNeil1, Frederik Claeyssens1
1Department of Materials Science and Engineering, University of Sheffield, 2Department of Chemistry, University of Sheffield, 3L. V. Prasad Eye Institute
We report a technique for the fabrication of micropockets within electrospun membranes in which to study cell behavior. Specifically, we describe a combination of microstereolithography and electrospinning for the production of PLGA (Poly(lactide-co-glycolide)) corneal biomaterial devices equipped with microfeatures.
<h2 class="jove_title"><a href="https://www.jove.com/video/51714">Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo</a></h2>
Juha T. Huiskonen, Marie-Laure Parsy, Sai Li, David Bitto, Max Renner, Thomas A. Bowden
Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
An approach is presented for determining structures of viral membrane glycoprotein complexes using a combination of electron cryo-tomography and sub-tomogram averaging with the computational package Jsubtomo.
<h2 class="jove_title"><a href="https://www.jove.com/video/51743">Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology</a></h2>
William S. Phipps*1, Zhizhong Yin*1, Candice Bae1, Julia Z. Sharpe1, Andrew M. Bishara2, Emily S. Nelson3, Aaron S. Weaver3, Daniel Brown4, Terri L. McKay3, DeVon Griffin3, Eugene Y. Chan1
1DNA Medicine Institute, 2Harvard Medical School, 3NASA Glenn Research Center, 4ZIN Technologies
Spaceflight blood diagnostics need innovation. Few demonstrations have been published illustrating in-flight, reduced-gravity health diagnostic technology. Here we present a method for construction and operation of a parabolic flight test rig for a prototype point-of-care flow-cytometry design, with components and preparation strategies adaptable to other setups.

december 2014: jove's year in review

In this Year in Review, we showcase some of our favorite video articles of the year 2014 in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
In <a href="http://www.jove.com/archive/83/january-2014">January</a>, <a href="http://www.jove.com/chem">JoVE Chemistry</a> revisited one of the most famous experiments of the 20th Century. Stanley Miller and his thesis advisor, Harold Urey, made scientific history in 1953 when they showed that under the right conditions, simple compounds in a flask could spontaneously form the building blocks of life. Over six decades later, <a href="http://www.jove.com/video/51039/conducting-miller-urey-experiments">Parker et al.</a> have demonstrated how to recreate the classic Miller-Urey experiment-combining water with various gases, And subjecting the mixture to sparks to simulate Earth's conditions 3 &#189; billion years ago. The resulting primordial soup contains various amino acids that are fundamental to life on earth.
<a href="http://www.jove.com/archive/84/february-2014">February</a>'s <a href="http://www.jove.com/neuro">Neuroscience</a> section featured the nematode worm C. elegans. This simple organism is easy to manipulate genetically. And it's sensitive to various compounds, so <a href="http://www.jove.com/video/50864">Hao and Buttner</a> [scientists can] use them in chemical genetic screens to study the mechanisms of antipsychotic drugs. 
In <a href="http://www.jove.com/archive/85/march-2014">March</a>, <a href="http://www.jove.com/ctm">JoVE Clinical and Translational Medicine</a> had an article on amyotrophic lateral sclerosis (ALS). This neurodegenerative disease affected baseball legend Lou Gehrig, and in 2014, inspired millions of people to dump ice buckets on their heads to raise money and awareness for ALS. An early sign of ALS is weakness in specific hand muscles, so <a href="http://www.jove.com/video/51056">Menon and Vucic</a> showed how recording motor responses from hand muscle groups can help diagnose this disease.
<a href="http://www.jove.com/archive/86/april-2014">April</a>'s edition of <a href="http://www.jove.com/enviro">JoVE Environment</a> featured a technique for expressing recombinant genes in plants. <a href="http://www.jove.com/video/51234/">Mattozzi et al.</a> delivered special expression constructs using a gene gun to specifically target recombinant genes to different subcellular organelles in plants. 
In <a href="http://www.jove.com/archive/87/may-2014">May</a>, <a href="http://www.jove.com/ctm">JoVE Clinical &amp; Translational Medicine</a>, <a href="http://www.jove.com/video/51476/">Thompson et al.</a> used coordinate mapping to analyze the pharyngeal phase of swallowing, a technique that can be useful for studying swallowing disorders. 
In <a href="http://www.jove.com/archive/88/june-2014">June</a>, <a href="http://www.jove.com/ap">JoVE Applied Physics</a> took us back in time again with a protocol that approximates conditions in the early Solar System. <a href="http://www.jove.com/video/51541">Blum et al.</a> examined collision forces between particles, which can aggregate over hundreds of millions of years to form planets. 
In the <a href="http://www.jove.com/archive/89/july-2014">July</a> edition of <a href="http://www.jove.com/behavior">JoVE Behavior</a>, <a href="http://www.jove.com/video/51605">Tung et al.</a> demonstrated standard behavioral tests for noninvasively assessing balance performance in mice. 
In <a href="http://www.jove.com/archive/90/august-2014">August</a>, <a href="http://www.jove.com/video/51669">Malide et al.</a> demonstrated in <a href="http://www.jove.com/biology">JoVE Biology</a> how to label hematopoietic precursor cells with different fluorescent markers so the cells can be tracked as they differentiate and engraft into bone marrow after transplantation.
In our <a href="http://www.jove.com/archive/91/september-2014">September</a> issue, <a href="http://www.jove.com/bioengineering">JoVE Bioengineering</a> featured innovative methods for treating the cornea, the clear front window of the eye. <a href="http://www.jove.com/video/51826">Ortega et al.</a> engineered membranes that could potentially regenerate the corneal epithelium and preserve vision in certain eye diseases.
In <a href="http://www.jove.com/archive/92/october-2014">October</a>, <a href="http://www.jove.com/i2">JoVE Immunology &amp; Infection</a> featured a technique for analyzing glycoprotein spikes on viral surfaces, which many viruses use to penetrate host cells. <a href="http://www.jove.com/video/51714">Huiskonen et al.</a> use a computational approach to study the precise, 3D structures of these spikes, which can guide the design of antiviral drugs and vaccines.
<a href="http://www.jove.com/archive/93/november-2014">November</a>'s edition of <a href="http://www.jove.com/biology">JoVE Biology</a> took <a href="http://www.jove.com/search?q=flow+cytometry">flow cytometry</a> to a whole new level with a protocol that can be performed in space. <a href="http://www.jove.com/video/51743">Phipps et al.</a> demonstrate the procedure on a parabolic flight, also known as a "vomit comet," which creates a weightless environment and presents a whole new set of challenges to laboratory research. 
And coming up in <a href="http://www.jove.com/archive/94">December</a>, we pinch off 2014 with a article describing fecal transplantation, When stool from a healthy donor is transferred to a patient infected with Clostridium difficile through a standard colonoscopy, it is a highly effective therapy for infections that don't respond to other treatments.
This Year in Review was just a sampling of <a href="http://www.jove.com/search?from=2014-01-01&amp;to=2014-12-31">more than 900 video articles</a> that JoVE produced for 2014. Browse the <a href="http://www.jove.com/search">JoVE archives</a> for thousands of other videos, and come back each week to see brand-new material in <a href="http://www.jove.com">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about December 2014: JoVE's Year in Review at JoVE.com

December 2014: JoVE&#039;s Year in Review

December 2014: JoVE's Year in Review

In this Year in Review, we showcase some of our favorite video articles of the year 2014 in JoVE: The Journal of Visualized Experiments.
In January, JoVE ...

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2.0K ビュー.  Massachusetts Eye and Ear.  

ビデオ: December 2014: JoVE's Year in Review

assessing dyslexia at six year of age

Assessing Dyslexia at Six Year of Age

This research sets out a proposed protocol for the identification of dyslexia. The protocol is based on diagnostic and response to intervention models. The proposal involves using structured interviews and standardized tests for the assessment of reading and writing performance and determinant...

classical short-delay eyeblink conditioning in one-year-old children

Classical Short-Delay Eyeblink Conditioning in One-Year-Old Children

This protocol describes an eyeblink conditioning paradigm appropriate for experiments with one-year-old infants. Commercial or custom-made equipment can be used to deliver the stimuli, and data collection and analysis should be performed on the video recordings.

scale-up chemical synthesis of thermally-activated delayed fluorescence emitters based on the dibenzothiophene-s,s-dioxide core

Scale-up Chemical Synthesis of Thermally-activated Delayed Fluorescence Emitters Based on the Dibenzothiophene-S,S-Dioxide Core

Scale-up synthesis of highly efficient thermally activated delayed fluorescence emitters is described in the presented...

high-throughput screening for protein-based inheritance in s. cerevisiae

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

This protocol describes a high-throughput methodology to functionally screen for protein-based inheritance in S.

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

colorimetric analysis of alkaline phosphatase activity in s. aureus biofilm

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

In this manuscript, we established a high throughput method to quantify alkaline phosphatase activity in S. aureus biofilm culture from 96-well tissue culture...

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

assay for adhesion and agar invasion in s. cerevisiae

Assay for Adhesion and Agar Invasion in S. cerevisiae

We describe a qualitative assay for yeast adhesion and agar invasion as a measure of invasive and pseudohyphal differentiation. This simple assay can be used to assess the invasive phenotype of various mutants as well as the effects environmental cues and signaling pathways on yeast...

point-of-care ultrasound: a review of ultrasound parameters for predicting difficult airways

Point-of-Care Ultrasound: A Review of Ultrasound Parameters for Predicting Difficult Airways

A point-of-care ultrasound (POCUS) is a simple, non-invasive, and portable tool that enables dynamic airway assessment. Several studies have attempted to determine the role of ultrasound parameters as an adjunct to clinical examination in predicting difficult...

equipment setup and artifact removal for simultaneous electroencephalogram and functional magnetic resonance imaging for clinical review in epilepsy

Equipment Setup and Artifact Removal for Simultaneous Electroencephalogram and Functional Magnetic Resonance Imaging for Clinical Review in Epilepsy

This article details simultaneous electroencephalogram and functional magnetic resonance imaging (EEG-fMRI) recording procedures that can be used in both clinical and research settings. EEG processing procedures to remove imaging artifacts for clinical review are also included. This study focuses on the example of epilepsy during the interictal...

systematic review and meta-analysis on the efficacy of angong niuhuang pill in viral encephalitis

Systematic Review and Meta-Analysis on the Efficacy of Angong Niuhuang Pill in Viral Encephalitis

laser micro-irradiation to study dna recruitment during s phase

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

This protocol describes a non-invasive method to efficiently identify S-phase cells for downstream microscopy studies, such as measuring DNA repair protein recruitment by laser...

identification of protein complexes with quantitative proteomics in s. cerevisiae

Identification of protein complexes with quantitative proteomics in S. cerevisiae

Here we describe a new quantitative proteomics technique for identifying protein complexes in Saccharomyces cerevisiae. In this study, we have used the SILAC method together with affinity purification followed by tandem mass spectrometry to identify with high specificity the binding partners of an ER protein,...

spectrophotometric screening for potential inhibitors of cytosolic glutathione s-transferases

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases

Glutathione S-transferases (GSTs) are detoxification enzymes involved in the metabolism of numerous chemotherapeutic drugs. Overexpression of GSTs is correlated with cancer chemotherapy resistance. One way to counter this phenotype is to use inhibitors. This protocol describes a method using a spectrophotometric assay to screen for potential GST...

determination of self-(in)compatibility and inter-(in)compatibility relationships in citrus using manual pollination, microscopy, and s-genotype analyses

Determination of Self-Incompatibility and Inter-Incompatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

This protocol provides a rapid method for determining pollen compatibility and incompatibility in citrus...

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

https://cloudfront.jove.com/CDNSource/teasers/65056.jpg

behavioral assessment of hearing in 2 to 4 year-old children: a two-interval, observer-based procedure using conditioned play-based responses

Behavioral Assessment of Hearing in 2 to 4 Year-old Children: A Two-interval, Observer-based Procedure Using Conditioned Play-based Responses

This paper describes a procedure for measuring hearing sensitivity in 2 to 4 year-old children. Children are trained to perform play-based responses when they hear a target signal. Thresholds are then estimated in a two-interval, two-alternative forced-choice paradigm, based on observations of the child&#39;s...

synthesis of terpolymers at mild temperatures using dynamic sulfur bonds in poly(s-divinylbenzene)

Synthesis of Terpolymers at Mild Temperatures Using Dynamic Sulfur Bonds in PolyS-Divinylbenzene

The goal of this protocol is to initiate polymerization using dynamic sulfur bonds in poly(S-divinylbenzene) at mild temperatures (90 &#176;C) without using solvents. Terpolymers are characterized by GPC, DSC and 1H NMR, and tested for changes in...

Synthesis of Terpolymers at Mild Temperatures Using Dynamic Sulfur Bonds in Poly(S-Divinylbenzene)

determination of s-phase duration using 5-ethynyl-2'-deoxyuridine incorporation in saccharomyces cerevisiae

Determination of S-Phase Duration Using 5-Ethynyl-2&#039;-deoxyuridine Incorporation in Saccharomyces cerevisiae

We describe two complementary protocols to accurately determine S-phase duration in S. cerevisiae using EdU, a thymidine analog, which is incorporated in vivo and detected using Click chemistry by microscopy and flow cytometry. It allows for the easy characterization of the duration of DNA replication and overlooked replication defects in...

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

a high-throughput method to globally study the organelle morphology in s. cerevisiae

A high-throughput method to globally study the organelle morphology in S. cerevisiae

GFP-fusion proteins are widely used to visualize organelles by confocal microscopy. However, screening for mutations that affect the morphology of organelles generally requires individual mutagenesis and is time consuming. Here, we demonstrate a method to simultaneously incorporate organelle-GFP markers in almost 5,000 non-essential genes in...

detection of protein s-acylation using acyl-resin assisted capture

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Acyl-RAC (Acyl-Resin Assisted Capture) is a highly sensitive, reliable and easy to perform method to detect reversible lipid modification of cysteine residues (S-acylation) in a variety of biological...

measuring mrna levels over time during the yeast s. cerevisiae hypoxic response

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular...

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

flow cytometry-based purification of s. cerevisiae zygotes

Flow Cytometry-based Purification of S. cerevisiae Zygotes

To purify zygotes of S. cerevisiae, haploid cells of opposite mating type were engineered to express red or green fluorescent proteins, co-incubated to allow zygote formation, and fractionated using a flow cytometry-based protocol. The highly-enriched fraction enables subsequent "-omic" studies, recovery of initial progeny, and systematic investigation of zygote...