<h2 class="jove_title"><a href="https://www.jove.com/video/52968">A Structured Rehabilitation Protocol for Improved Multifunctional Prosthetic Control: A Case Study</a></h2>
Aidan Dominic Roche1,2, Ivan Vujaklija3,4, Sebastian Ams&#252;ss3,4, Agnes Sturma1,5, Peter G&#246;bel6, Dario Farina3,4, Oskar C. Aszmann1,2
1Christian Doppler Laboratory for Restoration of Extremity Function, 2Department of Surgery, Division of Plastic and Reconstructive Surgery, Medical University of Vienna, 3Department of Neurorehabilitation Engineering, Bernstein Focus Neurotechnology G&#246;ttingen, 4University Medical Center G&#246;ttingen, Georg-August University, 5University of Applied Sciences FH Campus Wien, 6Research &amp; Development, Otto Bock Healthcare Products GmbH
As prosthetic development moves towards the goal of natural control, harnessing amputees&#8217; inherent ability to learn new motor skills may enable proficiency. This manuscript describes a structured rehabilitation protocol, which includes imitation, repetition, and reinforcement learning strategies, for improved multifunctional prosthetic control.
<h2 class="jove_title"><a href="https://www.jove.com/video/53242">Conditions Affecting Social Space in Drosophila melanogaster</a></h2>
Alison R. McNeil1, Sam N. Jolley1, Adesanya A. Akinleye2, Marat Nurilov2, Zulekha Rouzyi2, Austin J. Milunovich3, Moria C. Chambers3, Anne F. Simon1
1Department of Biology, University of Western Ontario, 2Department of Biology, York College/CUNY, 3Department of Entomology, Cornell University
The effect of genes and environment on social space of Drosophila melanogaster can be quantified through a powerful but straightforward analytical paradigm. We show here different factors that can affect this social space, and thus need to be taken into consideration when designing experiments in this paradigm.
<h2 class="jove_title"><a href="https://www.jove.com/video/53184">Thermal Imaging to Study Stress Non-invasively in Unrestrained Birds</a></h2>
Paul Jerem, Katherine Herborn, Dominic McCafferty, Dorothy McKeegan, Ruedi Nager
Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary &#38; Life Sciences, University of Glasgow
There is a need for a non-invasive assessment of stress. This paper describes a simple protocol using thermal imaging to detect a significant response in eye-region temperature in wild blue tits to a mild acute stressor.

november 2015 - this month in jove: drosophila social space, structured rehabilitation for multifunctional prosthetics, and thermal imaging in wild birds

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/105">November 2015 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
In <a href="http://www.jove.com/neuro">JoVE Neuroscience</a>, we know that <a href="http://www.jove.com/science-education/5082/an-introduction-to-drosophila-melanogaster">fruit flies (Drosophila melanogaster)</a>are a lot like humans in many ways-especially because they like their personal space. And in fruit flies, this preferred social distance can be measured using the social space assay. <a href="http://www.jove.com/video/53242/">McNiel et al.</a> demonstrate this straightforward protocol, which requires only simple equipment and experimental setups. Flies are blown into a social chamber and forced to form a tight group. Then they're allowed to take their preferred distance from one another. These distances are measured and processed with free online software (ImageJ). This social space assay provides a simple yet powerful paradigm for analyzing the underlying neurogenetics and environmental factors of social behavior.
In <a href="http://www.jove.com/behavior">JoVE Behavior</a>, humans have a natural ability to acquire new motor skills, and this ability is crucial for upper limb amputees as they learn the complex control schemes for advanced multifunctional prosthetics. This month, <a href="http://www.jove.com/video/52968">Roche et al.</a> present a case study of a structured rehabilitation method, which aims to improve multifunctional prosthetic control. Their subject underwent a structured protocol of imitation, repetition, and reinforcement learning. The subject demonstrated improvement in a widely used hand function test. This study suggests that a structured rehabilitation method may facilitate proficiency for multifunctional prosthetic control, and provides basis for larger clinical studies.
<a href="http://www.jove.com/science-education/5429/modeling-social-stress">Stress</a> is a major concept in <a href="http://www.jove.com/behavior">JoVE Behavior</a>, and comprises various physiological responses to challenges. Among other responses, stress increases body temperature, which provides a quantitative measure of this response. However, the very act of measuring body temperature can be stressful to subjects, especially if they're wild animals. So <a href="http://www.jove.com/video/53184/">Jerem et al.</a> present a protocol for noninvasively measuring temperature in wild birds using infrared thermography. Their set-up is equipped with bird food and an infrared camera. This takes a thermal video of the bird before and after the researcher remotely closes the box, which acts as a mild acute stressor. The skin around the bird's eye is the warmest area in the image, and this protocol provides a time series of eye-region temperature with fine temporal resolution. With further validation, this method may prove valuable for studying the dynamics of the stress response for a wide range of researchers from environmental science to medicine. 
You've just had a sneak peek of the <a href="http://www.jove.com/archive/105">November 2015 issue</a> of <a href="http://www.jove.com/">JoVE</a>. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about November 2015 - This Month in JoVE: Drosophila Social Space, Structured Rehabilitation for Multifunctional Prosthetics, and Thermal Imaging in Wild Birds at J

November 2015 - This Month in JoVE: Drosophila Social Space, Structured Rehabilitation for Multifunctional Prosthetics, and Thermal Imaging in Wild Birds

Here's a look at what's coming up in the November 2015 issue of JoVE: The Journal of Visualized Experiments.
In JoVE Neuroscience, we know that fruit ...

november-2015-this-month-jove-drosophila-social-space-structured

Without Section

1.8K ビュー.  Massachusetts Eye and Ear. November 2015 - This Month in JoVE: Drosophila Social Space, Structured Rehabilitation for Multifunctional Prosthetics, and Thermal Imaging in Wild Birds

ビデオ: November 2015 - This Month in JoVE: Drosophila Social Space, Structured Rehabilitation for Multifunctional Prosthetics, and Thermal Imaging in Wild Birds

a structured rehabilitation protocol for improved multifunctional prosthetic control: a case study

A Structured Rehabilitation Protocol for Improved Multifunctional Prosthetic Control: A Case Study

As prosthetic development moves towards the goal of natural control, harnessing amputees&#8217; inherent ability to learn new motor skills may enable proficiency. This manuscript describes a structured rehabilitation protocol, which includes imitation, repetition, and reinforcement learning strategies, for improved multifunctional prosthetic...

conditions affecting social space in drosophila melanogaster

Conditions Affecting Social Space in Drosophila melanogaster

The effect of genes and environment on social space of Drosophila melanogaster can be quantified through a powerful but straightforward analytical paradigm. We show here different factors that can affect this social space, and thus need to be taken into consideration when designing experiments in this...

Conditions Affecting Social Space in Drosophila melanogaster

thermal imaging to study stress non-invasively in unrestrained birds

Thermal Imaging to Study Stress Non-invasively in Unrestrained Birds

There is a need for a non-invasive assessment of stress. This paper describes a simple protocol using thermal imaging to detect a significant response in eye-region temperature in wild blue tits to a mild acute...

social space assay to explore social behavior defects in the drosophila model of autism spectrum disorder

Social Space Assay to Explore Social Behavior Defects in the Drosophila Model of Autism Spectrum Disorder

structured motor rehabilitation after selective nerve transfers

Structured Motor Rehabilitation After Selective Nerve Transfers

Here, we present a protocol for the motor rehabilitation of patients with severe nerve injuries and selective nerve transfer surgery. It aims at restoring the motor function proposing several stages in patient education, early-stage therapy after surgery and interventions for rehabilitation after successful re-innervation of the nerve&#8217;s...

straightforward assay for quantification of social avoidance in drosophila melanogaster

Straightforward Assay for Quantification of Social Avoidance in Drosophila melanogaster

Here, we present a protocol to quantify the avoidance of stressed individuals. This paradigm is powerful yet user-friendly and can be used to assess the influence of genes and environment on one kind of social interaction in Drosophila...

Straightforward Assay for Quantification of Social Avoidance in Drosophila melanogaster

rapid diagnosis of avian influenza virus in wild birds: use of a portable rrt-pcr and freeze-dried reagents in the field

Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field

This study describes diagnosis of avian influenza in wild birds using a portable rRT-PCR system. The method takes advantage of freeze-dried reagents to screen wild birds in a non-laboratory setting, typical of an outbreak scenario. Use of molecular tools provides accurate and sensitive alternatives for rapid...

paradigms for behavioral assessment in drosophila model of autism spectrum disorder

Author Spotlight: Exploring Autism Spectrum Disorder Symptoms in Fruit Flies &amp;#8212; Genetic Models and Behavioral Tests

Autism Spectrum Disorder (ASD) is associated with impaired social and communicative behavior and the emergence of repetitive behavior. For studying the interrelation between ASD genes and behavioral deficits in the Drosophila model, five behavioral paradigms are described in this paper for assaying social spacing, aggression, courtship, grooming, and habituation behavior.

&lt;i&gt;Drosophila&lt;/i&gt; Models for Genetic and Behavioral Traits of Autism Spectrum Disorder

https://cloudfront.jove.com/CDNSource/teasers/66649_b.jpg

local and global methods of assessing thermal nociception in drosophila larvae

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

In this article, we demonstrate assays to study thermal nociception in Drosophila larvae. One assay involves spatially-restricted (local) stimulation of thermal nociceptors1,2 while the second involves a wholesale (global) activation of most or all such neurons3. Together, these techniques allow visualization and quantification of the behavioral functions of Drosophila nociceptive sensory...

Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae

an automated method to determine the performance of drosophila in response to temperature changes in space and time

An Automated Method to Determine the Performance of Drosophila in Response to Temperature Changes in Space and Time

Here we present a protocol to automatically determine the locomotor performance of Drosophila at changing temperatures using a programmable temperature-controlled arena that produces fast and accurate temperature changes in time and space.

An Automated Method to Determine the Performance of Drosophila in Response to Temperature Changes in Space and Time

nuclei isolation and super-resolution structured illumination microscopy for examining nucleoporin alterations in human neurodegeneration

Nuclei Isolation and Super-Resolution Structured Illumination Microscopy for Examining Nucleoporin Alterations in Human Neurodegeneration

This protocol describes an optimized workflow for nuclei isolation and super-resolution structured illumination microscopy to evaluate individual nucleoporins within the nucleoplasm and NPCs in induced pluripotent stem cell derived neurons and postmortem human...

in vivo imaging of muscle-tendon morphogenesis in drosophila pupae

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae

Here, we present an easy-to-use and versatile method to perform live imaging of developmental processes in general and muscle-tendon morphogenesis in particular in living Drosophila...

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae

simple microfluidic devices for in vivo imaging of c. elegans, drosophila and zebrafish

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

A simple microfluidic device has been developed to perform anesthetic free in vivo imaging of C. elegans, intact Drosophila larvae and zebrafish larvae. The device utilizes a deformable PDMS membrane to immobilize these model organisms in order to perform time lapse imaging of numerous processes such as heart beat, cell division and sub-cellular neuronal transport.  We demonstrate the use of this device and show examples of different types of data collected from different model...

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

imaging calcium in drosophila at egg activation

Imaging Calcium in Drosophila at Egg Activation

This article describes an adaptable ex vivo protocol for visualizing Ca2+ during egg activation in...

Imaging Calcium in Drosophila at Egg Activation

using microfluidics chips for live imaging and study of injury responses in drosophila larvae

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

Drosophila larvae are an attractive model system for live imaging due to their translucent cuticle and powerful genetics. This protocol describes how to utilize a single-layer PDMS device, called the &#39;larva chip&#39; for live imaging of cellular processes within neurons of 3rd instar Drosophila...

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

mesoscopic fluorescence tomography for in-vivo imaging of developing drosophila

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

Mesoscopic  fluorescence tomography operates beyond the penetration limits of  tissue-sectioning fluorescence microscopy. The technique is based on  multi-projection illumination and a photon transport description. We  demonstrate in-vivo whole-body 3D visualization of the morphogenesis of  GFP-expressing wing imaginal discs in Drosophila...

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

live imaging and analysis of muscle contractions in drosophila embryo

Live Imaging and Analysis of Muscle Contractions in Drosophila Embryo

Here, we present a method to record embryonic muscle contractions in Drosophila embryos in a non-invasive and detail-oriented...

Live Imaging and Analysis of Muscle Contractions in Drosophila Embryo

the social dimension of stress: experimental manipulations of social support and social identity in the trier social stress test

The Social Dimension of Stress: Experimental Manipulations of Social Support and Social Identity in the Trier Social Stress Test

Previous research on the social dimension of stress has focused on two important variables: social identity and social support. This protocol introduces an effective experimental manipulation of these two social variables and describes their implementation in a standard stress induction paradigm (Trier Social Stress Test).

drosophila preparation and longitudinal imaging of heart function in vivo using optical coherence microscopy (ocm)

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy OCM

Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM...

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

dissection and imaging of active zones in the drosophila neuromuscular junction

Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction

The neuromuscular junction (NMJ) of Drosophila melanogaster is an important model system for studying normal synaptic function as well as perturbations to synaptic function found in certain neurological diseases. We present a protocol for dissection of the Drosophila larval motor system and immunostaining for active zone proteins within the...

Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Research

JoVE Journal

Biology

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...