<h2 class="jove_title"><a href="https://www.jove.com/video/54034">Spotting Cheetahs: Identifying Individuals by Their Footprints</a></h2>Zoe C. Jewell1,2, Sky K. Alibhai1,2, Florian Weise3,4, Stuart Munro3, Marlice Van Vuuren5, Rudie Van Vuuren51WildTrack 501(c)3 (wildtrack.org), 2Nicholas School of the Environment, Duke University, 3N&#8725;a&#8242;an ku s&#234; Research Programme, 4Division of Biology and Conservation Ecology, School of Science and the Environment, Manchester Metropolitan University, 5N&#8725;a&#8242;an ku s&#234; FoundationThe cheetah (Acinonyx jubatus) is an iconic, endangered species, but conservation efforts are challenged by habitat shrinkage and conflict with commercial farmers. The footprint identification technique, a robust, accurate and cost-effective image classification system, is a new approach to monitoring cheetahs.<h2 class="jove_title"><a href="https://www.jove.com/video/53773">An Experimental Analysis of Children's Ability to Provide a False Report about a Crime</a></h2>Joshua Wyman, Ida Foster, Victoria TalwarDepartment of Educational and Counselling Psychology, McGill UniversityThe current methodology is designed to provide an ecologically relevant approach for measuring the veracity, length and quality of children's true and false testimonies. Implications of the current methodology for future research and professionals who interview children will also be discussed.<h2 class="jove_title"><a href="https://www.jove.com/video/53986">Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses</a></h2>Catherine Cheng, David S. Gokhin, Roberta B. Nowak, Velia M. FowlerDepartment of Cell and Molecular Biology, The Scripps Research InstituteAge-related increases in eye lens stiffness are linked to presbyopia. This protocol describes a simple, cost-effective method for measuring mouse lens stiffness. Mouse lenses, like human lenses, become stiffer with age. This method is precise and can be adapted for lenses from larger animals.<h2 class="jove_title"><a href="https://www.jove.com/video/53963">Digital Printing of Titanium Dioxide for Dye Sensitized Solar Cells</a></h2>Ruth Cherrington, Benjamin Michael Wood, Iulia Salaoru, Vannessa GoodshipWarwick Manufacturing Group, University of WarwickThis paper investigates the suitability of inkjet printing for the manufacturing of dye-sensitized solar cells. A binder-free TiO2 nanoparticle ink was formulated and printed onto a FTO glass substrate. The printed layer was fabricated into a cell with an active area of 0.25 cm2 and an efficiency of 3.5%.

may 2016 - this month in jove: cheetah footprints, lens stiffness, and digitally printed solar cells

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/111">May 2016 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
In <a href="http://www.jove.com/enviro">JoVE Environment</a>, we examine the cheetah-one of Africa's most iconic animals, but also an endangered species. Monitoring the cheetah population is challenging to conservation efforts, especially since current tagging techniques can be invasive, expensive, and unreliable. <a href="http://www.jove.com/video/54034/">Jewell et al.</a> present a new approach that uses image classification of footprints to identify individual cheetahs. The footprint identification algorithms can distinguish cheetahs from other species (due to unique species-specific anatomy) and predict individual cheetah identity with high accuracy. This is the first robust footprint identification technique described for cheetahs and is applicable at any site where footprints are found. 
In <a href="http://www.jove.com/med">JoVE Medicine</a>, the lens of the eye owes much of its focusing power on its ability to change shape so it can form clear images on the retina. In humans, age-related stiffening of the lens leads to a reduced ability to accommodate or focus on close objects. Like human lenses, mouse lenses become stiffer with age, so <a href="http://www.jove.com/video/53986">Cheng et al.</a> use them as a model for understanding lens pathologies. They present a protocol for determining mouse lens stiffness, applying sequentially increasing compressive loads onto the lens. This method is precise, simple, and reproducible, and can be scaled up to mechanically test lenses from larger animals.
In <a href="http://www.jove.com/behavior">JoVE Behavior</a>, we feature an experimental analysis of children's capacity to tell lies. In police and forensic interviews, kids have been known to purposely omit information, and even make false denials or accusations. In this study, <a href="http://www.jove.com/video/53773">Wyman et al.</a> have children witness a staged theft, and then ask them to either falsely deny the theft, falsely accuse someone else of the theft, or tell the truth. They measure various aspects of both true and false accounts, and examine the social and developmental factors that influence a child's testimony. These methods can help improve the understanding of testimonies from children.
In <a href="http://www.jove.com/eng">JoVE Engineering</a>, scientists are developing alternatives for conventional silicon solar cells, because their production is expensive and consumes a lot of energy. This month, <a href="http://www.jove.com/video/53963">Cherrington et al.</a> present methods for the large-scale production of dye-sensitized solar cells (DSSCs) using inkjet printing. DSSCs have a fundamentally different working principle than silicon solar cells, and their production is less damaging to the environment. With continued development, this inkjet-printing process may be a low-cost, environmentally friendly alternative to conventional solar cells.
You've just had a sneak peek of the <a href="http://www.jove.com/archive/111">May 2016 issue</a> of <a href="http://www.jove.com/">JoVE</a>. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about May 2016 - This Month in JoVE: Cheetah footprints, lens stiffness, and digitally printed solar cells at JoVE.com

May 2016 - This Month in JoVE: Cheetah footprints, lens stiffness, and digitally printed solar cells

Here's a look at what's coming up in the May 2016 issue of JoVE: The Journal of Visualized Experiments.
In JoVE Environment, we examine the cheetah-one of ...

may-2016-this-month-jove-cheetah-footprints-lens-stiffness-digitally

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1.5K ビュー.  Massachusetts Eye and Ear. May 2016 - This Month in JoVE: Cheetah footprints, lens stiffness, and digitally printed solar cells

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spotting cheetahs: identifying individuals by their footprints

Spotting Cheetahs: Identifying Individuals by Their Footprints

The cheetah (Acinonyx jubatus) is an iconic, endangered species, but conservation efforts are challenged by habitat shrinkage and conflict with commercial farmers. The footprint identification technique, a robust, accurate and cost-effective image classification system, is a new approach to monitoring...

automated compression testing of the ocular lens

Author Spotlight: Advancing Understanding of Age-Related Lens Stiffness Changes 

We present an automated method for characterizing the effective elastic modulus of an ocular lens using a compression...

Biomechanical Characterization of Porcine Ocular Lens with Automated Compression

https://cloudfront.jove.com/CDNSource/teasers/66040_b.jpg

sequential application of glass coverslips to assess the compressive stiffness of the mouse lens: strain and morphometric analyses

Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses

Age-related increases in eye lens stiffness are linked to presbyopia. This protocol describes a simple, cost-effective method for measuring mouse lens stiffness. Mouse lenses, like human lenses, become stiffer with age. This method is precise and can be adapted for lenses from larger...

digital printing of titanium dioxide for dye sensitized solar cells

Digital Printing of Titanium Dioxide for Dye Sensitized Solar Cells

This paper investigates the suitability of inkjet printing for the manufacturing of dye-sensitized solar cells. A binder-free TiO2 nanoparticle ink was formulated and printed onto a FTO glass substrate. The printed layer was fabricated into a cell with an active area of 0.25 cm2 and an efficiency of...

printing fabrication of bulk heterojunction solar cells and in situ morphology characterization

Printing Fabrication of Bulk Heterojunction Solar Cells and In Situ Morphology Characterization

Here, we present a protocol to fabricate organic thin film solar cells using a mini-slot die coater and related in-line structure characterizations using synchrotron scattering...

Printing Fabrication of Bulk Heterojunction Solar Cells and In Situ Morphology Characterization

in situ monitoring of the accelerated performance degradation of solar cells and modules: a case study for cu(in,ga)se2 solar cells

In Situ Monitoring of the Accelerated Performance Degradation of Solar Cells and Modules: A Case Study for CuIn,GaSe2 Solar Cells

Two &#39;Combined Stress test with in situ measurement&#39; setups, which allow real-time monitoring of accelerated degradation of solar cells and modules, were designed and constructed. These setups allow the simultaneous use of humidity, temperature, electrical biases, and illumination as independently controlled stress factors. The setups and various experiments executed are...

In Situ Monitoring of the Accelerated Performance Degradation of Solar Cells and Modules: A Case Study for Cu(In,Ga)Se2 Solar Cells

optimizing mouse primary lens epithelial cell culture: a comprehensive guide to trypsinization

Author Spotlight: Unlocking the Secrets of Cataracts &amp;#8211; Investigating Redox Repair Enzymes in Lens Epithelial Cells

This manuscript outlines a detailed video protocol for culturing primary lens epithelial cells (LECs), aiming to improve reproducibility and aid research in cataracts and posterior capsule opacification (PCO). It offers step-by-step instructions on lens dissection, LECs isolation, and validation, serving as a valuable guide, especially for newcomers in the...

Cultivation and Characterization of Lens Epithelial Cells

https://cloudfront.jove.com/CDNSource/teasers/65912_b.jpg

well-aligned vertically oriented zno nanorod arrays and their application in inverted small molecule solar cells

Well-aligned Vertically Oriented ZnO Nanorod Arrays and their Application in Inverted Small Molecule Solar Cells

This manuscript describes how to design and fabricate efficient inverted SMPV1:PC71BM solar cells with ZnO nanorods (NRs) grown on a high quality Al-doped ZnO (AZO) seed layer. The well-aligned vertically oriented ZnO NRs exhibit high crystalline properties. The power conversion efficiency of solar cells can reach...

integration of light trapping silver nanostructures in hydrogenated microcrystalline silicon solar cells by transfer printing

Integration of Light Trapping Silver Nanostructures in Hydrogenated Microcrystalline Silicon Solar Cells by Transfer Printing

A viable transfer printing-based methodology to introduce plasmonic metal nanostructures in solar cells is described.  Using nanopillar poly(dimethylsiloxane) stamps, an Ag-based ordered nanodisk array was integrated with standard hydrogenated microcrystalline Si solar cells, which led to improved device performances due to plasmonic light...

monovalent cation doping of ch3nh3pbi3 for efficient perovskite solar cells

Monovalent Cation Doping of CH3NH3PbI3 for Efficient Perovskite Solar Cells

Here, we present a protocol to adjust the properties of solution-processed CH3NH3PbI3 through the incorporation of monovalent cation additives in order to achieve highly efficient perovskite solar...

Monovalent Cation Doping of CH3NH3PbI3 for Efficient Perovskite Solar Cells

indacenodithienothiophene-based ternary organic solar cells: concept, devices and optoelectronic analysis

In this study, in addition to the synthesis of a novel polymer, we fully characterize a ternary bulk-heterojunction solar cell, with a power conversion efficiency exceeding 4.6%, with the complementary use of optical and electrical...

Indacenodithienothiophene-Based Ternary Organic Solar Cells: Concept, Devices and Optoelectronic Analysis

developing high performance gap/si heterojunction solar cells

Developing High Performance GaP/Si Heterojunction Solar Cells

Here, we present a protocol to develop high-performance GaP/Si heterojunction solar cells with a high Si minority-carrier...

inkjet-printed polyvinyl alcohol multilayers

Inkjet-printed Polyvinyl Alcohol Multilayers

An inkjet printer was used to manufacture polyvinyl alcohol multilayers. Polyvinyl alcohol water-based ink was formulated, and the main physical properties were investigated.

may-grunwald giemsa staining: a method to stain bone marrow cells

This video describes the technique of staining bone marrow cells using May- Gr&#252;nwald Giemsa Staining. In the protocol, we will stain the murine myeloid leukemia cells, C149 cells, to analyze the morphology and differential...

May-Grunwald Giemsa Staining: A Method to Stain Bone Marrow Cells

electrospinning of photocatalytic electrodes for dye-sensitized solar cells

Electrospinning of Photocatalytic Electrodes for Dye-sensitized Solar Cells

The overall goal of this project was to use electrospinning to fabricate a photoanode with improved performance for dye-sensitized solar...

flash infrared annealing for perovskite solar cell processing

Flash Infrared Annealing for Perovskite Solar Cell Processing

We describe a flash infrared annealing method used for the synthesis of perovskite and mesoscopic-TiO2 films. Annealing parameters are varied and optimized for processing on fluorine-doped tin oxide (FTO) glass and indium tin oxide-coated polyethylene terephthalate (ITO PET), subsequently giving devices power conversion efficiencies...

fabrication of robust nanoscale contact between a silver nanowire electrode and cds buffer layer in cu(in,ga)se2 thin-film solar cells

Fabrication of Robust Nanoscale Contact between a Silver Nanowire Electrode and CdS Buffer Layer in CuIn,GaSe2 Thin-film Solar Cells

In this protocol, we describe the detailed experimental procedure for the fabrication of a robust nanoscale contact between a silver nanowire network and CdS buffer layer in a CIGS thin-film solar...

Fabrication of Robust Nanoscale Contact between a Silver Nanowire Electrode and CdS Buffer Layer in Cu(In,Ga)Se2 Thin-film Solar Cells

isolating mouse lens epithelial cells to study lens-related disorders

Isolating Mouse Lens Epithelial Cells to Study Lens-Related Disorders

identification of footprints of rna:protein complexes via rna immunoprecipitation in tandem followed by sequencing (ripit-seq)

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Here, we present a protocol to enrich endogenous RNA binding sites or &#34;footprints&#34;&#160;of RNA:protein (RNP) complexes from mammalian cells. This approach involves two immunoprecipitations of RNP subunits and is therefore dubbed RNA immunoprecipitation in tandem...

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

morphology control for fully printable organic&#8211;inorganic bulk-heterojunction solar cells based on a ti-alkoxide and semiconducting polymer

Morphology Control for Fully Printable Organic&amp;#8211;Inorganic Bulk-heterojunction Solar Cells Based on a Ti-alkoxide and Semiconducting Polymer

A method for fully printable, fullerene-free, highly air-stable, bulk-heterojunction solar cells based on Ti alkoxides as the electron acceptor and the electron-donating polymer fabrication is described here. Moreover, a method for controlling the morphology of the photoactive layer through the molecular bulkiness of the Ti-alkoxide units is...

Morphology Control for Fully Printable Organic&#8211;Inorganic Bulk-heterojunction Solar Cells Based on a Ti-alkoxide and Semiconducting Polymer

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Research

JoVE Journal

Biology

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...