Department of Translational Molecular Pathology
I am an Associate Professor and Director of the Multiplex Immunofluorescence and Image Analysis Laboratory in the Department of Translational Molecular Pathology at The University of Texas MD Anderson Cancer Center. I obtained my M.D. from the University of Mayor de San Andres, Bolivia, and I finished my Residence in Pathology at Sao Paulo Medical School, Brazil. Then I obtained my Ph.D. and my first Postdoctoral Fellow at the same University and my second Postdoctoral Fellow at The University of Texas, MD Anderson Cancer Center. I was promoted to Research Scientist and, in a short period, to Assistant Professor at the same University. I am a pathologist with a strong background in surgical pathology and research. My main interest is tissue immune profiling and elucidating the mechanisms involved in the pathogenesis and progression of cancer, particularly lung cancer. I aimed to accomplish this objective by applying various staining techniques and imaging platforms, including multiplex immunofluorescence and RNAscope, and high-plex imaging platforms, such as CO-Detection by indEXing and multiplexed ion beam imaging. I am also interested in studying the immune response to immunotherapy in longitudinal cancer studies to discover and characterize new biomarkers for future treatment approaches. I have over 15 years of research experience teaching technicians, graduate students, and doctoral and postdoctoral fellows. I also have extensive experience in performing standard laboratory techniques. I authored over 100 articles on lung cancer, interstitial lung diseases in human and animal models, and immune profiling using the various image analysis–based methodologies described above. I have also authored several abstracts and presentations for national and international scientific meetings, book chapters, and reports on pulmonary pathology and other medical topics.
4-1BB Agonist Focuses CD8(+) Tumor-Infiltrating T-Cell Growth into a Distinct Repertoire Capable of Tumor Recognition in Pancreatic Cancer.
Clinical cancer research : an official journal of the American Association for Cancer Research Sep, 2017 | Pubmed ID: 28947567
Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues.
Scientific reports Oct, 2017 | Pubmed ID: 29042640
Immunohistochemical and Image Analysis-Based Study Shows That Several Immune Checkpoints are Co-expressed in Non-Small Cell Lung Carcinoma Tumors.
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 06, 2018 | Pubmed ID: 29526824
Effect of neoadjuvant chemotherapy on the immune microenvironment in non-small cell lung carcinomas as determined by multiplex immunofluorescence and image analysis approaches.
Journal for immunotherapy of cancer Jun, 2018 | Pubmed ID: 29871672
A Functional Spatial Analysis Platform for Discovery of Immunological Interactions Predictive of Low-Grade to High-Grade Transition of Pancreatic Intraductal Papillary Mucinous Neoplasms.
Cancer informatics , 2018 | Pubmed ID: 30013304
Histomorphometric evaluation of the Ki-67 proliferation rate and CD34 microvascular and D2-40 lymphovascular densities drives the pulmonary typical carcinoid outcome.
Human pathology 11, 2018 | Pubmed ID: 30031097
Usefulness of complementary next-generation sequencing and quantitative immunohistochemistry panels for predicting brain metastases and selecting treatment outcomes of non-small cell lung cancer.
Human pathology 01, 2019 | Pubmed ID: 30218756
State-of-the-Art of Profiling Immune Contexture in the Era of Multiplexed Staining and Digital Analysis to Study Paraffin Tumor Tissues.
Cancers Feb, 2019 | Pubmed ID: 30791580
B7-H3 Expression in Merkel Cell Carcinoma-Associated Endothelial Cells Correlates with Locally Aggressive Primary Tumor Features and Increased Vascular Density.
Clinical cancer research : an official journal of the American Association for Cancer Research 06, 2019 | Pubmed ID: 30808776
Targeting the Interplay between Epithelial-to-Mesenchymal-Transition and the Immune System for Effective Immunotherapy.
Cancers May, 2019 | Pubmed ID: 31137625
Multi-region exome sequencing reveals genomic evolution from preneoplasia to lung adenocarcinoma.
Nature communications 07, 2019 | Pubmed ID: 31278276
Multiplex Immunofluorescence Assays.
Methods in molecular biology (Clifton, N.J.) , 2020 | Pubmed ID: 31502166
Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies.
Cancers Jan, 2020 | Pubmed ID: 31972974
Comprehensive T cell repertoire characterization of non-small cell lung cancer.
Nature communications 01, 2020 | Pubmed ID: 32001676
Variants in Epithelial-Mesenchymal Transition and Immune Checkpoint Genes Are Associated With Immune Cell Profiles and Predict Survival in Non-Small Cell Lung Cancer.
Archives of pathology & laboratory medicine 10, 2020 | Pubmed ID: 32150457
Multi-omic molecular profiling reveals potentially targetable abnormalities shared across multiple histologies of brain metastasis.
Acta neuropathologica 02, 2021 | Pubmed ID: 33394124
Identification of distinct immune landscapes using an automated nine-color multiplex immunofluorescence staining panel and image analysis in paraffin tumor tissues.
Scientific reports 02, 2021 | Pubmed ID: 33633208
Detection of Programmed Cell Death Ligand 1 Expression in Lung Cancer Clinical Samples by an Automated Immunohistochemistry System.
Methods in molecular biology (Clifton, N.J.) , 2021 | Pubmed ID: 33683684
Western Blot as a Support Technique for Immunohistochemistry to Detect Programmed Cell Death Ligand 1 Expression.
Methods in molecular biology (Clifton, N.J.) , 2021 | Pubmed ID: 33683685
Case Report: Enfortumab Vedotin for Metastatic Urothelial Carcinoma: A Case Series on the Clinical and Histopathologic Spectrum of Adverse Cutaneous Reactions From Fatal Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis to Dermal Hypersensitivity Reaction.
Frontiers in oncology , 2021 | Pubmed ID: 33747934
Pilot Clinical Trial of Perioperative Durvalumab and Tremelimumab in the Treatment of Resectable Colorectal Cancer Liver Metastases.
Clinical cancer research : an official journal of the American Association for Cancer Research 06, 2021 | Pubmed ID: 33811152
Immuno-profiling and cellular spatial analysis using five immune oncology multiplex immunofluorescence panels for paraffin tumor tissue.
Scientific reports 04, 2021 | Pubmed ID: 33875760
Contextual cues from cancer cells govern cancer-associated fibroblast heterogeneity.
Cell reports 04, 2021 | Pubmed ID: 33882319
Author Correction: Multi-region exome sequencing reveals genomic evolution from preneoplasia to lung adenocarcinoma.
Nature communications May, 2021 | Pubmed ID: 33980839
Multiplex Immunofluorescence Tyramide Signal Amplification for Immune Cell Profiling of Paraffin-Embedded Tumor Tissues.
Frontiers in molecular biosciences , 2021 | Pubmed ID: 33996912
Methods to Determine and Analyze the Cellular Spatial Distribution Extracted From Multiplex Immunofluorescence Data to Understand the Tumor Microenvironment.
Frontiers in molecular biosciences , 2021 | Pubmed ID: 34179080
A proteogenomic portrait of lung squamous cell carcinoma.
Cell 08, 2021 | Pubmed ID: 34358469
Pathology Quality Control for Multiplex Immunofluorescence and Image Analysis Assessment in Longitudinal Studies.
Frontiers in molecular biosciences , 2021 | Pubmed ID: 34395517
Best Practices for Technical Reproducibility Assessment of Multiplex Immunofluorescence.
Frontiers in molecular biosciences , 2021 | Pubmed ID: 34532339
Single cell T cell landscape and T cell receptor repertoire profiling of AML in context of PD-1 blockade therapy.
Nature communications 10, 2021 | Pubmed ID: 34663807
Cold and heterogeneous T cell repertoire is associated with copy number aberrations and loss of immune genes in small-cell lung cancer.
Nature communications 11, 2021 | Pubmed ID: 34789716
Effector memory cytotoxic CD3/CD8/CD45RO T cells are predictive of good survival and a lower risk of recurrence in triple-negative breast cancer.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc Nov, 2021 | Pubmed ID: 34839351
Impact of Region-of-Interest Size on Immune Profiling Using Multiplex Immunofluorescence Tyramide Signal Amplification for Paraffin-Embedded Tumor Tissues.
Pathobiology : journal of immunopathology, molecular and cellular biology May, 2022 | Pubmed ID: 35609532
Pathological Response and Immune Biomarker Assessment in Non-Small-Cell Lung Carcinoma Receiving Neoadjuvant Immune Checkpoint Inhibitors.
Cancers Jun, 2022 | Pubmed ID: 35681755
Multiplex Immunofluorescence and the Digital Image Analysis Workflow for Evaluation of the Tumor Immune Environment in Translational Research.
Frontiers in oncology , 2022 | Pubmed ID: 35832550
Durvalumab plus tremelimumab in advanced or metastatic soft tissue and bone sarcomas: a single-centre phase 2 trial.
The Lancet. Oncology Sep, 2022 | Pubmed ID: 35934010
Immunogenomic profiling of lung adenocarcinoma reveals poorly differentiated tumors are associated with an immunogenic tumor microenvironment.
Lung cancer (Amsterdam, Netherlands) Oct, 2022 | Pubmed ID: 35973335
Editorial: Multiplexed image analysis for translational research project applications.
Frontiers in oncology , 2022 | Pubmed ID: 36465347
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