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Fujian Key Laboratory of Organ and Tissue Regeneration

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Optimization of the Cuff Technique for Murine Heart Transplantation

Yunhan Ma^*1,2, Baiyi Xie^*1,2, Helong Dai^*3,4,5, Chenxi Wang⁶, Shujiao Liu⁶, Tianshu Lan⁷, Shuangyue Xu^1,2, Guoliang Yan^1,2,6, Zhongquan Qi^1,2,8

¹Organ Transplantation Institute, School of Medicine, Xiamen University, ²Fujian Key Laboratory of Organ and Tissue Regeneration, ³Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, ⁴Clinical Research Center for Organ Transplantation in Hunan Province, ⁵Clinical Immunology Center, Central South University, ⁶School of Medicine, Xiamen University, ⁷Xiamen Medical College, ⁸Medical College, Guangxi University

We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time.

Bioengineering

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Phillip Kyriakakis¹, Lourdes Fernandez de Cossio², Patrick Wade Howard¹, Sivleng Kouv¹, Marianne Catanho¹, Vincent J. Hu³, Robert Kyriakakis¹, Molly E. Allen¹, Yunhan Ma⁴, Marcelo Aguilar-Rivera¹, Todd P. Coleman¹

¹Department of Bioengineering, University of California, San Diego, ²University of California, San Diego, ³Department of Mathematical Computational, and Systems biology, University of California, Irvine, ⁴Department of Biomedical Engineering, Duke University

This protocol describes how to perform optogenetic experiments for controlling gene expression with red and far-red light using PhyB and PIF3. Included are step-by-step instructions for building a simple and flexible illumination system, which enables the control of gene expression or other optogenetics with a computer.