Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of MinipigsWon-Jae Lee 1,2, Ji-Sung Park 1, Si-Jung Jang 1, Seung-Chan Lee 1, HyeonJeong Lee 1, Jae-Hoon Lee 1, Gyu-Jin Rho 1, Sung-Lim Lee 1
1College of Veterinary Medicine, Gyeongsang National University, 2PWG Genetics Pte Ltd.
A protocol establishing mesenchymal stem cells (MSCs) isolated from the synovial fluid and bone marrow of minipigs and non-invasively collected using syringe aspiration is presented. Cellular phenotyping was performed using flow cytometry after cell isolation and in vitro cultivation of bone marrow and synovial fluids derived from MSCs.
