murine alveolar bone specimen collection 

골격 기계 생물학을 탐구하기 위한 폐포골 리모델링 분석

It is generally known that bone is under constant reconstruction throughout life, in response to mechanical forces according to Wolff&#39;s law1,2. Appropriate mechanical stimulation, such as gravity and daily exercise, maintains bone mass and strength and prevents bone loss by stimulating both osteoblasts and osteoclasts. Osteoclasts, responsible for bone resorption3,4,5,6,7, and osteoblasts, responsible for bone formation8,9,10, maintain bone homeostasis and function jointly in the biological process of bone remodeling. In contrast, in the absence of loading stimuli, as in astronauts under long-term microgravity, bones suffer 10% bone mineral density loss, thus increasing the risk of osteoporosis11,12. Furthermore, noninvasive and convenient mechanical therapies, including orthodontics and distraction osteogenesis, have emerged as treatments for bone diseases13,14. All these have shown that mechanical force plays a critical role in maintaining bone quality and quantity. Recent studies generally analyzed bone remodeling in response to mechanical loading using time-consuming models such as running wheel and tail suspension tests, which usually took 4 weeks or more to simulate force loading or unloading15,16. Therefore, there is demand for a convenient and efficient animal model for studying bone remodeling driven by force loading.
The alveolar bone is the most active in terms of bone remodeling, with a high turnover rate17. Orthodontic tooth movement (OTM), a common treatment for malocclusion, is an artificial process of alveolar bone remodeling in response to mechanical force. However, OTM, which induces rapid bone remodeling18, is also a time-saving way to study the effects of mechanical force on bone remodeling compared with other models with a long experimental period. Therefore, OTM is an ideal model to study bone remodeling under mechanical stimuli. It is noteworthy that the mechanism of alveolar bone remodeling is often time-sensitive, and it is necessary to observe the changes in alveolar bone remodeling at certain time points after modeling. With the dual advantages of temporal and spatial control of DNA recombination and tissue specificity, an inducible conditional gene knockout mouse model is a suitable choice for OTM studies.
Conventionally, OTM-mediated alveolar bone remodeling has been divided into tension zones involving bone formation and pressure zones involving bone resorption19,20,21, which is more detailed but difficult to regulate. Furthermore, Yuri et al. reported that the time of bone formation in OTM differed on the tension and compression sides22. In addition, a previous study had demonstrated that the first molar could initiate wide remodeling of the maxillary alveolar bone under orthodontic force, which was not constrained to the tension and pressure zones23. Therefore, we selected the area located within three roots of M1 in the cross-section of the maxillary bone as the region of interest (ROI) and described methods to assess the activity of osteoblasts and osteoclasts in the same area to evaluate alveolar bone remodeling under OTM.
As a nuclear transcription factor, signal transducer and activator of transcription 3 (STAT3) has been proven critical in bone homeostasis24,25. Previous studies have reported low bone mineral density and recurrent pathological fractures in Stat3-mutant mice26,27. Our previous study demonstrated that deletion of Stat3 in Osx+ osteoblasts caused craniofacial malformation and osteoporosis, as well as spontaneous bone fracture28. Recently, we provided in vivo evidence with an inducible osteoblast-specific Stat3 deletion mouse model (Col1&#945;2CreERT2; Stat3fl/fl, hereafter called Stat3Col1&#945;2ERT2) that STAT3 is critical in mediating the effects of orthodontic force driving alveolar bone remodeling29. In this study, we provide methods and protocols for using inducible osteoblast lineage-specific Stat3 knockout mice to study bone remodeling under orthodontic force and describe methods for analyzing alveolar bone remodeling during OTM, thus shedding light on skeletal mechanical biology.

저자 스포트라이트: 폐포와 장골 리모델링을 비교하여 OTM 모델 잠재력 탐색 

유도 가능한 조골 계통 특이적 Stat3 녹아웃 마우스를 사용하여 교정 치아 운동 중 치조골 리모델링 연구

Our research focuses on the mechanism of bone remodeling under mechanical force, including alveolar bone and long bones. We try to answer how bones respond to mechanical stimuli to shed new light on skeletal mechanical biology for further clinical treatment. Currently, studies on OTM were limited to gene expression analysis in vivo or cellular function analysis in vitro.To this end, a challenge arose. How to trace and explore the function of specific genes in specific lineages during OTM in vivo? That's why we use inducible conditional knockout mice here.Orthodontic tooth movement, which induces rampant bone remodeling, is a time-saving method to study the effects of mechanical force on bone remodeling compared to other models that have a long experimental period. Our protocol also reveals dynamic alveolar bone remodeling at different time points with inducible gene-specific knockout mice. There are many important cell lineages in the alveolar bone.We aim to trace them and find out their underlying mechanisms during OTM. Furthermore, we will compare the mechanisms between alveolar bone and long bone remodeling to provide further clues for the potential of OTM model in biomechanical research.

Watch this Scientific Journal Video about Murine Alveolar Bone Specimen Collection at JoVE.com

Murine Alveolar Bone Specimen Collection   

안락사된 쥐의 자궁경부 부위에서 피부를 수직으로 잘라내고 머리와 몸통을 분리하고 머리의 전체 피부를 해부합니다. 다음으로, 양측 앙굴루스 오리스에서 하악 후방까지 피부와 복근을 잘라냅니다. 코라코이드에서 협측 근육과 힘줄을 완전히 분리하여 하악골을 제거합니다.완전한 상악골을 얻기 위해 여분의 뼈를 다듬습니다. 양측 제3대구치 뒤의 뼈를 제거하고 구개 점막을 벗겨냅니다. 마지막으로 교정 장치를 분리하고 정중 구개 봉합사를 따라 앞니 사이의 뼈를 잘라 좌우 치조골을 얻습니다.조골세포의 STAT3 결실에서 치조골 리모델링은 치조골의 면역형광 염색에 의해 확인되었습니다. 실체현미경 검사는 야생형 쥐의 치아 교정 이동 거리가 4일, 7일, 10일에 증가한 것으로 나타났다. 그러나 STAT3 녹아웃 마우스에서 교정 치아 이동 거리가 감소했습니다.10일째 Micro-CT 분석에서 움직임이 감소한 것을 확인했으며, 이는 STAT3 결실이 교정 치아 움직임을 늦추는 것을 나타냅니다.

Research

JoVE Journal

Medicine

180 조회수.  Shanghai Jiao Tong University School of Medicine. 안락사된 쥐의 자궁경부 부위에서 피부를 수직으로 잘라내고 머리와 몸통을 분리하고 머리의 전체 피부를 해부합니다. 다음으로, 양측 앙굴루스 오리스에서 하악 후방까지 피부와 복근을 잘라냅니다. 코라코이드에서 협측 근육과 힘줄을 완전히 분리하여 하악골을 제거합니다.완전한 상악골을 얻기 위해 여분의 뼈를 다듬습니다. 양측 제3대구치 뒤의 뼈를 제거하고 구?...

비디오: Murine Alveolar Bone Specimen Collection   

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

The alveolar bone, with a high turnover rate, is the most actively-remodeling bone in the body. Orthodontic tooth movement (OTM) is a common artificial process of alveolar bone remodeling in response to mechanical force, but the underlying mechanism remains elusive. Previous studies have been unable to reveal the precise mechanism of bone remodeling in any time and space due to animal model-related restrictions. The signal transducer and activator of transcription 3 (STAT3) is important in bone metabolism, but its role in osteoblasts during OTM is unclear. To provide in vivo evidence that STAT3 participates in OTM at specific time points and in particular cells during OTM, we generated a tamoxifen-inducible osteoblast lineage-specific Stat3 knockout mouse model, applied orthodontic force, and analyzed the alveolar bone phenotype. 
Micro-computed tomography (Micro-CT) and stereo microscopy were used to access OTM distance. Histological analysis selected the area located within three roots of the first molar (M1) in the cross-section of the maxillary bone as the region of interest (ROI) to evaluate the metabolic activity of osteoblasts and osteoclasts, indicating the effect of orthodontic force on alveolar bone. In short, we provide a protocol for using inducible osteoblast lineage-specific Stat3 knockout mice to study bone remodeling under orthodontic force and describe methods for analyzing alveolar bone remodeling during OTM, thus shedding new light on skeletal mechanical biology.

This study provides a protocol for using inducible osteoblast lineage-specific Stat3 knockout mice to study bone remodeling under orthodontic force and describes methods for analyzing alveolar bone remodeling during orthodontic tooth movement, thus shedding light on skeletal mechanical biology.

The alveolar bone, with a high turnover rate, is the most actively-remodeling bone in the body. Orthodontic tooth movement (OTM) is a common artificial ...

연구

의학

Murine Orthodontic Tooth Movement (OTM) Model

Begin by selecting 10 six-week old male mice and placing them into two groups labeled WT and CKO, with five mice in each group. Administer Tamoxifen to all the mice every two days for a week. Prepare a plastic mouse dissection platform as an operating table to immobilize the mice.Attach an elastic band to each rubber post for limb fixation. Tie another thread to the metal rod to hold the upper incisors. Place the anesthetized mouse in the supine position on the operating table.Use four elastic bands to fix the limbs. One thread attached to the metal rod to hold the upper incisors. Next, prepare closed coil springs for orthodontic force application.Ligate one end of the prepared spring to the maxillary left first molar. Ensure a stable force of 10 grams by measuring with the dynamometer. Then ligate the other end to the central incisor using a 0.1 millimeter resin reinforced steel ligature wire.After the operation, place the mice in a recovery cage. Monitor the mice for two to four hours until they regain sufficient consciousness to maintain sternal recumbency. Provide a soft diet and observe the mice regularly for complications, administering analgesic drugs as needed.

Murine Orthodontic Tooth Movement(OTM) 모델

Begin by cutting the skin vertically off the cervical region of the euthanized mice separate the head from the body, with the entire skin of the head dissected. Next, cut off the skin and buccinator muscles from the bilateral angulus oris to the posterior region of the mandible. Completely disconnect the buccal muscles and tendons from the coracoids to remove the mandible.Trim any extra bones to obtain the complete maxillae. Remove the bone behind the bilateral third molars, and peel off the palatal mucosa. Finally, disconnect the orthodontic appliance, and cut the bone between the incisors along the median palatine suture to obtain the right and left alveolar bone.Alveolar bone remodeling in the STAT3 deletion in osteoblasts was confirmed by immunofluorescent staining of alveolar bone. Stereomicroscopy revealed that the orthodontic tooth movement distance of the wild type mice increased on days four, seven, and 10. However, the orthodontic tooth movement distance was reduced in the STAT3 knockout mice.Micro-CT analysis on day 10 confirmed the reduced movement, indicating that STAT3 deletion decelerated orthodontic tooth movement.