To begin, fix the porcine lung tissue in 4%paraformaldehyde at room temperature for 48 hours. Then transfer the lung tissue to a 50 milliliter conical tube containing 30 milliliters of PBS with 0.01%Sodium Azide. Keep a plastic cup fine forceps and sterile scissors ready.
Heat the agarose in a microwave until it reaches boiling point. Then cool it down to 42 degrees Celsius in a water bath. Store it in the water bath until ready for use.
Remove the lung biopsy from the PBS Azide solution. Use fine forceps and sterile scissors to carefully dissect the lung lobes into smaller blocks. Next, cut down the middle of a plastic cup.
Pour a small amount of agarose at the bottom of the cup. Place the lid of a 50 milliliter conical tube with its rim removed on the agaro surface. Store this set up at four degrees Celsius for five minutes to allow the agarose to solidify.
Once the agarose has solidified, remove the plastic cup from the refrigerator. Now pour about one milliliter of liquid agarose onto the lid. Then gently place the lung tissue block on the lid.
Once the agarose has semis solidified, slowly submerged the lung tissue in liquid agarose. Refrigerate the setup at four degrees Celsius for about 15 minutes until the agarose becomes solid. Use a sharp blade to carefully remove any excess agarose surrounding the lung tissue.
