electrode fabrication for recording local field potentials in mouse hippocampus and prefrontal cortex

인지 연구 및 약물 분석을 위한 쥐 뇌의 LFP 기록

Local field potentials (LFPs) refer to the electrical activity recorded from the extracellular space, reflecting the collective activity of a localized group of neurons. They exhibit a diverse range of frequencies, spanning from slow waves at 1 Hz to fast oscillations at 100 Hz or 200 Hz. Specific frequency bands have been associated with cognitive functions such as learning, memory, and decision making1,2. Changes in LFP properties have been used as biomarkers for various neurological disorders, including dementia and schizophrenia3,4. Analyzing LFP recordings can offer valuable insights into the underlying pathological mechanisms associated with these conditions and potential therapeutic strategies.
Dual LFP recording is a technique used to measure localized electrical activity within and between two specific brain regions. This technique provides a valuable opportunity to investigate the intricate neural dynamics and signal communication occurring within and between distinct brain regions. Prior studies have revealed that detecting alterations in the neuronal properties of individual brain regions can be complex, but changes in interregional cortical communication can be observed5,6. Therefore, the utilization of dual LFP recording offers a potent means to address this issue.
Hippocampal-prefrontal connectivity plays a crucial role in modulating cognitive functions, and dysfunction has been linked to various neurological disorders7,8. Dual electrode recordings of these regions can provide information about these interactions. Unfortunately, there is limited information available on methods for performing dual electrode LFP recordings between these areas. Moreover, commercially available recording devices are generally expensive and lack adaptability to specific experimental designs. The conventional method for recording LFPs involves using a shielded cable to connect the recording device to electrodes implanted in an animal's brain. However, this approach is susceptible to motion artifacts and environmental noise, impacting the quality and reliability of the recorded signals.
This protocol describes a comprehensive procedure for performing dual-electrode LFP recordings in the mouse hippocampus and prefrontal cortex, using a low-cost custom-designed headstage that can be placed on the animal's head. These methods enable researchers to investigate region-specific oscillatory patterns within two discrete cerebral regions and explore interregional information exchange and connectivity between these areas.

저자 스포트라이트: 생쥐에서 이중 LFP 기록 프로토콜을 사용하여 뇌 신호에 대한 약물 영향 연구

Mouse Hippocampus and Prefrontal Cortex의 Dual Extracellular Recordings in the Mouse Hippocampus and Prefrontal Cortex(생쥐 해마와 전전두엽 피질의 이중 세포외 기록)

This study presents a comprehensive protocol for performing dual-electrode LFP recordings in the mouse hippocampus and the prefrontal cortex to investigate the effects of drugs on LFP properties in these areas. This protocol provides a low-cost and ultra-light brain signal recording device that is fully optimized for cognitive research in mice. This study provides a means to record signals with high signal-noise ratios in different brain regions, facilitating the investigation of interregional information communication within the brain.

Electrode Fabrication for Recording Local Field Potentials in mouse hippocampus and prefrontal cortex(마우스 해마와 전전두엽 피질의 국소자기장 전위를 기록하기 위한 전극 제작)

시작하려면 폴리테트라플루오로에틸렌 코팅된 텅스텐 와이어를 전전두엽 피질, 해마 및 접지 전극의 특정 길이로 자릅니다. 황동 튜브를 3mm 세그먼트로 자릅니다. 라이터를 사용하여 각 와이어 끝에 있는 코팅 2mm를 제거한 다음 전극 와이어를 황동 튜브에 단단히 납땜합니다.접지 전극의 경우 M1 0.2 스테인리스강 나사를 전극에 납땜하고 인산 기반 플럭스를 나사에 적용하여 납땜을 향상시킵니다.

electrode-fabrication-for-recording-local-field-potentials-mouse

Research

JoVE Journal

Neuroscience

Electrode Fabrication for Recording Local Field Potentials in Mouse Hippocampus and Prefrontal Cortex

100 조회수.  The University of Melbourne. 시작하려면 폴리테트라플루오로에틸렌 코팅된 텅스텐 와이어를 전전두엽 피질, 해마 및 접지 전극의 특정 길이로 자릅니다. 황동 튜브를 3mm 세그먼트로 자릅니다. 라이터를 사용하여 각 와이어 끝에 있는 코팅 2mm를 제거한 다음 전극 와이어를 황동 튜브에 단단히 납땜합니다.접지 전극의 경우 M1 0.2 스테인리스강 나사를 전극에 납땜하고 인산 기반 플럭스를 나사에 적?...

비디오: Electrode Fabrication for Recording Local Field Potentials in mouse hippocampus and prefrontal cortex(마우스 해마와 전전두엽 피질의 국소자기장 전위를 기록하기 위한 전극 제작)

Dual Extracellular Recordings in the Mouse Hippocampus and Prefrontal Cortex

The technique of recording local field potentials (LFPs) is an electrophysiological method used to measure the electrical activity of localized neuronal populations. It serves as a crucial tool in cognitive research, particularly in brain regions like the hippocampus and prefrontal cortex. Dual LFP recordings between these areas are of particular interest as they allow the exploration of interregional signal communication. However, methods for performing these recordings are rarely described, and most commercial recording devices are either expensive or lack adaptability to accommodate specific experimental designs. This study presents a comprehensive protocol for performing dual-electrode LFP recordings in the mouse hippocampus and the prefrontal cortex to investigate the effects of antipsychotic drugs and potassium channel modulators on LFP properties in these areas. The technique enables the measurement of LFP properties, including power spectra within each brain region and coherence between the two. Additionally, a low-cost, custom-designed recording device has been developed for these experiments. In summary, this protocol provides a means to record signals with high signal-to-noise ratios in different brain regions, facilitating the investigation of interregional information communication within the brain.

This protocol outlines the use of a custom-designed recording device and electrodes to record local field potentials and investigate information flow in the mouse hippocampus and prefrontal cortex.

The technique of recording local field potentials (LFPs) is an electrophysiological method used to measure the electrical activity of localized neuronal ...