Magnetic Separation of Vascular Wall-Resident CD34⁺ Stem Cells Isolated from Murine Aortal and Mesenteric Arteries for Cardiovascular Studies

To begin, isolate and culture the outer layer of the aorta and the first branch of the mesenteric arteries isolated from the anesthetized mouse. Once the cells become 80%confluent, centrifuge the dissociated cell suspension at 300G for five minute