Preparation of Serum Samples for Stability Evaluation of Human Adenosine Deaminase 1 Enzyme Using Microplate Assay

To begin, prepare a 10x stock of HsADA1 in 1x PBS at pH 7.4 to achieve a final concentration of 10 micromolar. Prewarm a thawed 15-milliliter aliquot of pooled human serum to 37 degrees Celsius. Then add 100 microliters of the 10x HsADA1 stock to