September 4th, 2019
•CRISPR-Cas9 technology provides an efficient method to precisely edit the mammalian genome in any cell type and represents a novel means to perform genome-wide genetic screens. A detailed protocol discussing the steps required for the successful performance of pooled genome-wide CRISPR-Cas9 screens is provided here.
Related Videos
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells
Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Copyright © 2024 MyJoVE Corporation. 판권 소유