JoVE Logo
교수 리소스 센터

로그인

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

DOI :

10.3791/61784-v

February 8th, 2021

February 8th, 2021

6,893 Views

1Biophysics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health

We describe a single-molecule approach to antigen-antibody affinity measurements using mass photometry (MP). The MP-based protocol is fast, accurate, uses a very small amount of material, and does not require protein modification.

Tags

Antibody antigen Affinity

-- Views

Related Videos

article

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

article

A Rapid Laser Probing Method Facilitates the Non-invasive and Contact-free Determination of Leaf Thermal Properties

article

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

article

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

article

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

article

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

article

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

article

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

article

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

article

Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

JoVE Logo

개인 정보 보호

이용 약관

정책

연구

교육

JoVE 소개

Copyright © 2024 MyJoVE Corporation. 판권 소유