April 1st, 2017
•Here we present protocols for affinity purification of protein complexes and their separation by blue native PAGE, followed by protein correlation profiling using label free quantitative mass spectrometry. This method is useful to resolve interactomes into distinct protein complexes.
Related Videos
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
Copyright © 2024 MyJoVE Corporation. 판권 소유