Growth Conditions for Nicotiana benthamiana Plants
3:05
Prepare Bacterial Strains for Agroinfiltration
6:20
Prepare Slides for Fluorescence Visualization
6:45
Fret-Flim Analysis Using a Confocal Laser Scanning Microscope
10:51
Fret-Flim Analysis Using a Confocal Laser Scanning Microscope: The Tripartite Interaction
12:31
Representative Results
13:42
Conclusion
필기록
The study of protein-protein interactions provides an understanding of the regulation of many biological processes. Here we demonstrate a procedure described initial by Y John shoe and co. Workers in 2007, where the authors have used BiFC in combi
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Here we present, a method to visualize ternary complex formation between three protein partners using fluorescent-tagged proteins by BiFC based FRET-FLIM assay. This method is valuable for studying protein-protein interaction complexes in vivo.