Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM AssayNeelima Boora *1, Vibha Verma *1, Ridhi Khurana 1, Gautam Gawande 1, Sanchi Bhimrajka 1, Komal Chaprana 1, Meenu Kapoor 2, Sanjay Kapoor 1
1Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, 2University School of Biotechnology, Guru Gobind Singh Indraprastha University
Here we present, a method to visualize ternary complex formation between three protein partners using fluorescent-tagged proteins by BiFC based FRET-FLIM assay. This method is valuable for studying protein-protein interaction complexes in vivo.
